Presence of asparagine‐linked <i>N‐</i>acetylglucosamine and chitobiose in <i>Pyrus pyrifolia</i> S‐RNases associated with gametophytic self‐incompatibility
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<jats:p>S‐RNases encoded by the <jats:italic>S‐</jats:italic>locus of rosaceous and solanaceous plants discriminate between the <jats:italic>S‐</jats:italic>alleles of pollen in gametophytic self‐incompatibility reactions, but it is not clear how. We report the structures of N‐glycans attached to each of the N‐glycosylation sites of seven S‐RNases in <jats:italic>Pyrus pyrifolia</jats:italic> of the Rosaceae. The structures were identified by chromatographic analysis of pyridylaminated sugar chains prepared from S<jats:sub>4</jats:sub>‐RNase and by liquid chromatography/electrospray ionization‐mass spectrometric analysis of the protease digests of reduced and S‐carboxymethylated S‐RNases. S<jats:sub>4</jats:sub>‐RNase carries various types of sugar chains, including plant‐specific ones with β1→2‐linked xylose and α1→3‐linked fucose residues. More than 70% of the total N‐glycans of S<jats:sub>4</jats:sub>‐RNase are, however, an <jats:italic>N‐</jats:italic>acetylglucosamine or a chitobiose (GlcNAcβ1→4GlcNAc), which has not been found naturally. The <jats:italic>N‐</jats:italic>acetylglucosamine and chitobiose are mainly present at the N‐glycosylation sites within the putative recognition sites of the S‐RNase, suggesting that these sugar chains may interact with pollen <jats:italic>S‐</jats:italic>product(s).</jats:p>
収録刊行物
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- European Journal of Biochemistry
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European Journal of Biochemistry 263 (3), 624-634, 1999-08
Wiley
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詳細情報
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- CRID
- 1363670320250386304
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- NII論文ID
- 80011230202
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- ISSN
- 14321033
- 00142956
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- データソース種別
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