Abortive expansion of the cumulus and impaired fertility in mice lacking the prostaglandin E receptor subtype EP <sub>2</sub>
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- Hiroko Hizaki
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Eri Segi
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Yukihiko Sugimoto
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Masaya Hirose
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Tomomi Saji
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Fumitaka Ushikubi
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Toshiyuki Matsuoka
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Yoichi Noda
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Takashi Tanaka
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Nobuaki Yoshida
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Shuh Narumiya
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
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- Atsushi Ichikawa
- Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan
抄録
<jats:p> Female mice lacking the gene encoding the prostaglandin (PG) E <jats:sub>2</jats:sub> receptor subtype EP <jats:sub>2</jats:sub> (EP <jats:sub>2</jats:sub> <jats:sup>−/−</jats:sup> ) become pregnant and deliver their pups at term, but with a much reduced litter size. A decrease in ovulation number and a much reduced fertilization rate were observed in EP <jats:sub>2</jats:sub> <jats:sup>−/−</jats:sup> females without difference of the uterus to support implantation of wild-type embryos. Treatment with gonadotropins induced EP <jats:sub>2</jats:sub> mRNA expression in the cumulus cells of ovarian follicles of wild-type mice. The immature cumuli oophori from wild-type mice expanded <jats:italic>in vitro</jats:italic> in response to both follicle-stimulating hormone and PGE <jats:sub>2</jats:sub> , but the response to PGE <jats:sub>2</jats:sub> was absent in those from EP <jats:sub>2</jats:sub> <jats:sup>−/−</jats:sup> mice. Cumulus expansion proceeded normally in preovulatory follicles but became abortive in a number of ovulated complexes in EP <jats:sub>2</jats:sub> <jats:sup>−/−</jats:sup> mice, indicating that EP <jats:sub>2</jats:sub> is involved in cumulus expansion in the oviduct <jats:italic>in vivo</jats:italic> . No difference in the fertilization rate between wild-type and EP <jats:sub>2</jats:sub> <jats:sup>−/−</jats:sup> mice was found in <jats:italic>in vitro</jats:italic> studies using cumulus-free oocytes. These results indicate that PGE <jats:sub>2</jats:sub> cooperates with gonadotropin to complete cumulus expansion for successful fertilization. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 96 (18), 10501-10506, 1999-08-31
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1364233270494555904
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- NII論文ID
- 80011327238
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- ISSN
- 10916490
- 00278424
- http://id.crossref.org/issn/00278424
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- データソース種別
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- Crossref
- CiNii Articles