Polarization of Chemoattractant Receptor Signaling During Neutrophil Chemotaxis

DOI Web Site 被引用文献20件
  • Guy Servant
    Department of Cellular and Molecular Pharmacology and
  • Orion D. Weiner
    Department of Cellular and Molecular Pharmacology and
  • Paul Herzmark
    Department of Cellular and Molecular Pharmacology and
  • Tamás Balla
    Endocrinology and Reproduction Research Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892–4510, USA.
  • John W. Sedat
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USA.
  • Henry R. Bourne
    Department of Cellular and Molecular Pharmacology and

抄録

<jats:p> Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the AKT protein kinase (or protein kinase B), tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils. Upon exposure of cells to chemoattractant, PHAKT-GFP is recruited selectively to membrane at the cell's leading edge, indicating an internal signaling gradient that is much steeper than that of the chemoattractant. Translocation of PHAKT-GFP is inhibited by toxin-B from <jats:italic>Clostridium difficile</jats:italic> , indicating that it requires activity of one or more Rho guanosine triphosphatases. </jats:p>

収録刊行物

  • Science

    Science 287 (5455), 1037-1040, 2000-02-11

    American Association for the Advancement of Science (AAAS)

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