Homologs of the yeast Sec complex subunits Sec62p and Sec63p are abundant proteins in dog pancreas microsomes

DOI Web Site 被引用文献3件
  • Jens Tyedmers
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
  • Monika Lerner
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
  • Christiane Bies
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
  • Johanna Dudek
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
  • Markus H. Skowronek
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
  • Ingrid G. Haas
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
  • Nicole Heim
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
  • Wolfgang Nastainczyk
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
  • Jörg Volkmer
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
  • Richard Zimmermann
    Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, D-66421 Homburg, Germany; and Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany

抄録

<jats:p>Cotranslational protein transport into dog pancreas microsomes involves the Sec61p complex plus a luminal heat shock protein 70. Posttranslational protein transport into the yeast endoplasmic reticulum (ER) involves the so-called Sec complex in the membrane, comprising a similar Sec61p subcomplex, the putative signal peptide receptor subcomplex, and the heat shock protein 40-type subunit, Sec63p, plus a luminal heat shock protein 70. Recently, human homologs of yeast proteins Sec62p and Sec63p were discovered. Here we determined the concentrations of these two membrane proteins in dog pancreas microsomes and observed that the canine homologs of yeast proteins Sec62p and Sec63p are abundant proteins, present in almost equimolar concentrations as compared with Sec61αp monomers. Furthermore, we detected fractions of these two proteins in association with each other as well as with the Sec61p complex. The J domain of the human Sec63p was shown to interact with immunoglobulin heavy chain binding protein. Thus, the membrane of the mammalian ER contains components, known from the posttranslationally operating protein translocase in yeast. We suggest that these components are required for efficient cotranslational protein transport into the mammalian ER as well as for other transport processes.</jats:p>

収録刊行物

被引用文献 (3)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ