The MITE family<i>Heartbreaker</i>(<i>Hbr</i>): Molecular markers in maize

DOI Web Site 被引用文献10件
  • Alexandra M. Casa
    Departments of Botany and Genetics, The University of Georgia, Athens, GA 30602; The Institute for Genomic Diversity and Department of Plant Breeding, Cornell University, Ithaca, NY 14853; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
  • Cory Brouwer
    Departments of Botany and Genetics, The University of Georgia, Athens, GA 30602; The Institute for Genomic Diversity and Department of Plant Breeding, Cornell University, Ithaca, NY 14853; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
  • Alexander Nagel
    Departments of Botany and Genetics, The University of Georgia, Athens, GA 30602; The Institute for Genomic Diversity and Department of Plant Breeding, Cornell University, Ithaca, NY 14853; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
  • Lianjiang Wang
    Departments of Botany and Genetics, The University of Georgia, Athens, GA 30602; The Institute for Genomic Diversity and Department of Plant Breeding, Cornell University, Ithaca, NY 14853; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
  • Qiang Zhang
    Departments of Botany and Genetics, The University of Georgia, Athens, GA 30602; The Institute for Genomic Diversity and Department of Plant Breeding, Cornell University, Ithaca, NY 14853; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
  • Stephen Kresovich
    Departments of Botany and Genetics, The University of Georgia, Athens, GA 30602; The Institute for Genomic Diversity and Department of Plant Breeding, Cornell University, Ithaca, NY 14853; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
  • Susan R. Wessler
    Departments of Botany and Genetics, The University of Georgia, Athens, GA 30602; The Institute for Genomic Diversity and Department of Plant Breeding, Cornell University, Ithaca, NY 14853; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131

抄録

<jats:p>Transposable elements are ubiquitous in plant genomes, where they frequently comprise the majority of genomic DNA. The maize genome, which is believed to be structurally representative of large plant genomes, contains single genes or small gene islands interspersed with much longer blocks of retrotransposons. Given this organization, it would be desirable to identify molecular markers preferentially located in genic regions. In this report, the features of a newly described family of miniature inverted repeat transposable elements (MITEs) (called<jats:italic>Heartbreaker</jats:italic>), including high copy number and polymorphism, stability, and preference for genic regions, have been exploited in the development of a class of molecular markers for maize. To this end, a modification of the AFLP procedure called transposon display was used to generate and display hundreds of genomic fragments anchored in<jats:italic>Hbr</jats:italic>elements. An average of 52 markers were amplified for each primer combination tested. In all, 213 polymorphic fragments were reliably scored and mapped in 100 recombinant inbred lines derived from a cross between the maize inbreds B73 × Mo17. In this mapping population,<jats:italic>Hbr</jats:italic>markers are distributed evenly across the 10 maize chromosomes. This procedure should be of general use in the development of markers for other MITE families in maize and in other plant and animal species where MITEs have been identified.</jats:p>

収録刊行物

被引用文献 (10)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ