Resetting of Circadian Time in Peripheral Tissues by Glucocorticoid Signaling

Aurélio Balsalobre, Steven A. Brown, Lysiane Marcacci, François Tronche, Christoph Kellendonk, Holger M. Reichardt, Günther Schütz, Ueli Schibler

doi:10.1126/science.289.5488.2344

Resetting of Circadian Time in Peripheral Tissues by Glucocorticoid Signaling

DOI Web Site 被引用文献121件

Aurélio Balsalobre

Département de Biologie Moléculaire, Sciences II, Université de Genève, 30 Quai Ernest Ansermet, CH-1211 Genève, Switzerland.
Steven A. Brown

Département de Biologie Moléculaire, Sciences II, Université de Genève, 30 Quai Ernest Ansermet, CH-1211 Genève, Switzerland.
Lysiane Marcacci

Département de Biologie Moléculaire, Sciences II, Université de Genève, 30 Quai Ernest Ansermet, CH-1211 Genève, Switzerland.
François Tronche

Molecular Biology of the Cell, Deutsches Krebsforschungzentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Federal Republic of Germany.
Christoph Kellendonk

Molecular Biology of the Cell, Deutsches Krebsforschungzentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Federal Republic of Germany.
Holger M. Reichardt

Molecular Biology of the Cell, Deutsches Krebsforschungzentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Federal Republic of Germany.
Günther Schütz

Molecular Biology of the Cell, Deutsches Krebsforschungzentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Federal Republic of Germany.
Ueli Schibler

Département de Biologie Moléculaire, Sciences II, Université de Genève, 30 Quai Ernest Ansermet, CH-1211 Genève, Switzerland.

抄録

<jats:p>In mammals, circadian oscillators reside not only in the suprachiasmatic nucleus of the brain, which harbors the central pacemaker, but also in most peripheral tissues. Here, we show that the glucocorticoid hormone analog dexamethasone induces circadian gene expression in cultured rat-1 fibroblasts and transiently changes the phase of circadian gene expression in liver, kidney, and heart. However, dexamethasone does not affect cyclic gene expression in neurons of the suprachiasmatic nucleus. This enabled us to establish an apparent phase-shift response curve specifically for peripheral clocks in intact animals. In contrast to the central clock, circadian oscillators in peripheral tissues appear to remain responsive to phase resetting throughout the day.</jats:p>