Efficient Initiation of HCV RNA Replication in Cell Culture

DOI Web Site 被引用文献44件
  • Keril J. Blight
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110–1093, USA.
  • Alexander A. Kolykhalov
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110–1093, USA.
  • Charles M. Rice
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110–1093, USA.

抄録

<jats:p>Hepatitis C virus (HCV) infection is a global health problem affecting an estimated 170 million individuals worldwide. We report the identification of multiple independent adaptive mutations that cluster in the HCV nonstructural protein NS5A and confer increased replicative ability in vitro. Among these adaptive mutations were a single amino acid substitution that allowed HCV RNA replication in 10% of transfected hepatoma cells and a deletion of 47 amino acids encompassing the interferon (IFN) sensitivity determining region (ISDR). Independent of the ISDR, IFN-α rapidly inhibited HCV RNA replication in vitro. This work establishes a robust, cell-based system for genetic and functional analyses of HCV replication.</jats:p>

収録刊行物

  • Science

    Science 290 (5498), 1972-1974, 2000-12-08

    American Association for the Advancement of Science (AAAS)

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