A maize cytokinin gene encoding an <i>O</i> -glucosyltransferase specific to <i>cis</i> -zeatin

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  • Ruth C. Martin
    Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University, Corvallis, OR 97331-7304; and Trait and Technology Development, Pioneer Hi-Bred International, Inc., Johnston, IA 50131-1004
  • Machteld C. Mok
    Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University, Corvallis, OR 97331-7304; and Trait and Technology Development, Pioneer Hi-Bred International, Inc., Johnston, IA 50131-1004
  • Jeffrey E. Habben
    Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University, Corvallis, OR 97331-7304; and Trait and Technology Development, Pioneer Hi-Bred International, Inc., Johnston, IA 50131-1004
  • David W. S. Mok
    Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University, Corvallis, OR 97331-7304; and Trait and Technology Development, Pioneer Hi-Bred International, Inc., Johnston, IA 50131-1004

抄録

<jats:p> Zeatin is a naturally occurring cytokinin. Biosynthesis and metabolism studies of zeatin have been directed mostly at the <jats:italic>trans</jats:italic> isomer, although <jats:italic>cis</jats:italic> -zeatin and its riboside occur as major components in some plant species. It is not known whether parallel regulatory pathways exist for the two isomers. Based on the sequence of the gene <jats:italic>ZOG1</jats:italic> encoding a <jats:italic>trans</jats:italic> -zeatin <jats:italic>O</jats:italic> -glucosyltransferase from <jats:italic>Phaseolus</jats:italic> (EC <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="ec" xlink:href="2.4.1.203">2.4.1.203</jats:ext-link> ), a <jats:italic>cis</jats:italic> -zeatin-specific <jats:italic>O</jats:italic> -glucosyltransferase was isolated from maize. This gene, <jats:italic>cisZOG1</jats:italic> , contains an ORF of 1,401 nucleotides encoding a protein of 51.1 kDa with 41% identity to the <jats:italic>Phaseolus</jats:italic> ZOG1 protein. Unexpectedly, the maize enzyme recognizes as substrates <jats:italic>cis</jats:italic> -zeatin and UDP-glucose but not <jats:italic>cis</jats:italic> -ribosylzeatin, <jats:italic>trans</jats:italic> -zeatin, or <jats:italic>trans</jats:italic> -ribosylzeatin. This finding indicates the existence of cis-specific regulatory elements in plants and suggests that <jats:italic>cis</jats:italic> -zeatin and derivatives may be more important in cytokinin homeostasis than currently recognized. </jats:p>

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