Characterization of a <i>Schizosaccharomyces pombe</i> mutant deficient in UDP‐galactose transport activity

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<jats:title>Abstract</jats:title><jats:p>In fission yeast, <jats:italic>Schizosaccharomyces pombe</jats:italic>, the carbohydrate components of the cell wall consist of galactomannan, unlike in <jats:italic>Saccharomyces cerevisiae</jats:italic>. We previously found that the disruption of <jats:italic>gms1</jats:italic><jats:sup>+</jats:sup>, a gene encoding the UDP‐galactose transporter required for the synthesis of galactomannan, led to the complete defect of cell surface galactosylation in <jats:italic>Sz. pombe</jats:italic>. The Δ<jats:italic>gms1</jats:italic> strain is therefore useful for the analysis of physiological properties of galactose residues in <jats:italic>Sz. pombe</jats:italic>. The deletion strain of <jats:italic>gms1</jats:italic><jats:sup>+</jats:sup> was viable; however, itshowed an aberrant cell morphology and increased sensitivities to digestion with β‐glucanase and to various drugs, such as hygromycin B, sodium orthovanadate and Calcofluor white. A reduction of galactomannan layers of the cell wall in the Δ<jats:italic>gms1</jats:italic> strain was observed by scanning and transmission electron microscopic analyses. The addition of osmotic stabilizer suppressed the morphologic defect of the Δ<jats:italic>gms1</jats:italic> cells, while other phenotypes were weakly suppressed. The Δ<jats:italic>gms1</jats:italic> (<jats:italic>h</jats:italic><jats:sup>90</jats:sup>) strain was incapable of sexual conjugation during nutritional starvation. These results suggest that the cell surface galactosylation is required not only for non‐sexual flocculation but also for sexual conjugation in <jats:italic>Sz. pombe</jats:italic>. Copyright © 2001 John Wiley & Sons, Ltd.</jats:p>

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  • Yeast

    Yeast 18 (10), 903-914, 2001-05-23

    Wiley

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