Determination of Pyrimidine Dimers in <i>Escherichia coli</i> and <i>Cryptosporidium parvum</i> during UV Light Inactivation, Photoreactivation, and Dark Repair

• Kumiko Oguma

  <!--label omitted: 1-->Department of Urban Engineering,1 and

• Hiroyuki Katayama

  <!--label omitted: 1-->Department of Urban Engineering,1 and

• Hiroshi Mitani

  <!--label omitted: 2-->Department of Integrated Biosciences,2 University of Tokyo, Bunkyo-ku, Tokyo, and

• Shigemitsu Morita

  <!--label omitted: 3-->College of Environmental Health, Azabu University, Sagamihara, Kanagawa,3 Japan

• Tsuyoshi Hirata

  <!--label omitted: 3-->College of Environmental Health, Azabu University, Sagamihara, Kanagawa,3 Japan

• Shinichiro Ohgaki

  <!--label omitted: 1-->Department of Urban Engineering,1 and

抄録

UV inactivation, photoreactivation, and dark repair of Escherichia coli and Cryptosporidium parvum were investigated with the endonuclease sensitive site (ESS) assay, which can determine UV-induced pyrimidine dimers in the genomic DNA of microorganisms. In a 99.9% inactivation of E. coli , high correlation was observed between the dose of UV irradiation and the number of pyrimidine dimers induced in the DNA of E. coli . The colony-forming ability of E. coli also correlated highly with the number of pyrimidine dimers in the DNA, indicating that the ESS assay is comparable to the method conventionally used to measure colony-forming ability. When E. coli were exposed to fluorescent light after a 99.9% inactivation by UV irradiation, UV-induced pyrimidine dimers in the DNA were continuously repaired and the colony-forming ability recovered gradually. When kept in darkness after the UV inactivation, however, E. coli showed neither repair of pyrimidine dimers nor recovery of colony-forming ability. When C. parvum were exposed to fluorescent light after UV inactivation, UV-induced pyrimidine dimers in the DNA were continuously repaired, while no recovery of animal infectivity was observed. When kept in darkness after UV inactivation, C. parvum also showed no recovery of infectivity in spite of the repair of pyrimidine dimers. It was suggested, therefore, that the infectivity of C. parvum would not recover either by photoreactivation or by dark repair even after the repair of pyrimidine dimers in the genomic DNA.

収録刊行物

• Applied and Environmental Microbiology

  Applied and Environmental Microbiology 67 (10), 4630-4637, 2001-10

  American Society for Microbiology

キーワード

• Ecology
• Applied Microbiology and Biotechnology
• Food Science
• Biotechnology

詳細情報

• CRID: 1363388846186556288
• NII論文ID: 80012775415
• DOI: 10.1128/aem.67.10.4630-4637.2001
• ISSN: 10985336
• ISSN: 00992240
• Web Site: https://journals.asm.org/doi/pdf/10.1128/AEM.67.10.4630-4637.2001
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