Distinct constitutive and low-CO ₂ -induced CO ₂ uptake systems in cyanobacteria: Genes involved and their phylogenetic relationship with homologous genes in other organisms

• Mari Shibata

  Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0814, Japan; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan; and Department of Plant Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

• Hiroshi Ohkawa

  Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0814, Japan; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan; and Department of Plant Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

• Takakazu Kaneko

  Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0814, Japan; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan; and Department of Plant Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

• Hideya Fukuzawa

  Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0814, Japan; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan; and Department of Plant Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

• Satoshi Tabata

  Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0814, Japan; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan; and Department of Plant Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

• Aaron Kaplan

  Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0814, Japan; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan; and Department of Plant Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

• Teruo Ogawa

  Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0814, Japan; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan; and Department of Plant Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

抄録

Cyanobacteria possess a CO 2 -concentating mechanism that involves active CO 2 uptake and HCO <jats:tex-math notation="LaTeX">\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{3}^{-}}}\end{equation*}\end{document} transport. For CO 2 uptake, we have identified two systems in the cyanobacterium Synechocystis sp. strain PCC 6803, one induced at low CO 2 and one constitutive. The low CO 2 -induced system showed higher maximal activity and higher affinity for CO 2 than the constitutive system. On the basis of speculation that separate NAD(P)H dehydrogenase complexes were essential for each of these systems, we reasoned that inactivation of one system would allow selection of mutants defective in the other. Thus, mutants unable to grow at pH 7.0 in air were recovered after transformation of a Δ ndhD3 mutant with a transposon-bearing library. Four of them had tags within slr1302 (designated cupB ), a homologue of sll1734 ( cupA ), which is cotranscribed with ndhF3 and ndhD3 . The Δ cupB, Δ ndhD4 , and Δ ndhF4 mutants showed CO 2 -uptake characteristics of the low CO 2 induced system observed in wild type. In contrast, mutants Δ cupA, Δ ndhD3 , and Δ ndhF3 showed characteristics of the constitutive CO 2 -uptake system. Double mutants impaired in one component of each of the systems were unable to take up CO 2 and required high CO 2 for growth. Phylogenetic analysis indicated that the ndhD3 / ndhD4 -, ndhF3 / ndhF4 -, and cupA / cupB -type genes are present only in cyanobacteria. Most of the cyanobacterial strains studied possess the ndhD3 / ndhD4- , ndhF3 / ndhF4- , and cupA / cupB -type genes in pairs. Thus, the two types of NAD(P)H dehydrogenase complexes essential for low CO 2 -induced and constitutive CO 2 -uptake systems associated with the NdhD3/NdhF3/CupA-homologues and NdhD4/NdhF4/CupB-homologues, respectively, appear to be present in these cyanobacterial strains but not in other organisms.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 98 (20), 11789-11794, 2001-09-18

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1364233270729288576
• NII論文ID: 80012775573
• DOI: 10.1073/pnas.191258298
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.191258298
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