O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

• J R Benson

• P E Hare

抄録

O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 72 (2), 619-622, 1975-02

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1362544418489655552
• NII論文ID: 80013171533
• DOI: 10.1073/pnas.72.2.619
• ISSN: 10916490
• ISSN: 00278424
• ISSN: http://id.crossref.org/issn/0015749X
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.72.2.619
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