Endophytic Colonization and In Planta Nitrogen Fixation by a <i>Herbaspirillum</i> sp. Isolated from Wild Rice Species

• Adel Elbeltagy

  <!--label omitted: 1-->Institute of Genetic Ecology, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577,1 and

• Kiyo Nishioka

  <!--label omitted: 1-->Institute of Genetic Ecology, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577,1 and

• Tadashi Sato

  <!--label omitted: 1-->Institute of Genetic Ecology, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577,1 and

• Hisa Suzuki

  <!--label omitted: 1-->Institute of Genetic Ecology, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577,1 and

• Bin Ye

  <!--label omitted: 1-->Institute of Genetic Ecology, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577,1 and

• Toru Hamada

  <!--label omitted: 2-->Marine Biotechnology Institute, Kamaishi Laboratories, Kamaishi City, Iwate 026-0001,2 Japan

• Tsuyoshi Isawa

  <!--label omitted: 1-->Institute of Genetic Ecology, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577,1 and

• Hisayuki Mitsui

  <!--label omitted: 1-->Institute of Genetic Ecology, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577,1 and

• Kiwamu Minamisawa

  <!--label omitted: 1-->Institute of Genetic Ecology, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577,1 and

抄録

Nitrogen-fixing bacteria were isolated from the stems of wild and cultivated rice on a modified Rennie medium. Based on 16S ribosomal DNA (rDNA) sequences, the diazotrophic isolates were phylogenetically close to four genera: Herbaspirillum , Ideonella , Enterobacter , and Azospirillum . Phenotypic properties and signature sequences of 16S rDNA indicated that three isolates (B65, B501, and B512) belong to the Herbaspirillum genus. To examine whether Herbaspirillum sp. strain B501 isolated from wild rice, Oryza officinalis , endophytically colonizes rice plants, the gfp gene encoding green fluorescent protein (GFP) was introduced into the bacteria. Observations by fluorescence stereomicroscopy showed that the GFP-tagged bacteria colonized shoots and seeds of aseptically grown seedlings of the original wild rice after inoculation of the seeds. Conversely, for cultivated rice Oryza sativa , no GFP fluorescence was observed for shoots and only weak signals were observed for seeds. Observations by fluorescence and electron microscopy revealed that Herbaspirillum sp. strain B501 colonized mainly intercellular spaces in the leaves of wild rice. Colony counts of surface-sterilized rice seedlings inoculated with the GFP-tagged bacteria indicated significantly more bacterial populations inside the original wild rice than in cultivated rice varieties. Moreover, after bacterial inoculation, in planta nitrogen fixation in young seedlings of wild rice, O. officinalis , was detected by the acetylene reduction and 15 N 2 gas incorporation assays. Therefore, we conclude that Herbaspirillum sp. strain B501 is a diazotrophic endophyte compatible with wild rice, particularly O. officinalis .

収録刊行物

• Applied and Environmental Microbiology

  Applied and Environmental Microbiology 67 (11), 5285-5293, 2001-11

  American Society for Microbiology

キーワード

• Ecology
• Applied Microbiology and Biotechnology
• Food Science
• Biotechnology

詳細情報

• CRID: 1362825893665603584
• NII論文ID: 80015197562
• DOI: 10.1128/aem.67.11.5285-5293.2001
• ISSN: 10985336
• ISSN: 00992240
• Web Site: https://journals.asm.org/doi/pdf/10.1128/AEM.67.11.5285-5293.2001
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