Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning

• Liangxue Lai

  Department of Animal Science,

• Donna Kolber-Simonds

  Immerge BioTherapeutics Inc., Charlestown, MA 02129, USA.

• Kwang-Wook Park

  Department of Animal Science,

• Hee-Tae Cheong

  Department of Animal Science,

• Julia L. Greenstein

  Immerge BioTherapeutics Inc., Charlestown, MA 02129, USA.

• Gi-Sun Im

  Department of Animal Science,

• Melissa Samuel

  Department of Animal Science,

• Aaron Bonk

  Department of Animal Science,

• August Rieke

  Department of Animal Science,

• Billy N. Day

  Department of Animal Science,

• Clifton N. Murphy

  Department of Animal Science,

• David B. Carter

  Department of Animal Science,

• Robert J. Hawley

  Immerge BioTherapeutics Inc., Charlestown, MA 02129, USA.

• Randall S. Prather

  Department of Animal Science,

抄録

The presence of galactose α-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.

収録刊行物

• Science

  Science 295 (5557), 1089-1092, 2002-02-08

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361418519985542912
• NII論文ID: 80015199796
• DOI: 10.1126/science.1068228
• ISSN: 10959203
• ISSN: 00368075
• ISSN: http://id.crossref.org/issn/00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.1068228
• データソース種別:
  • Crossref
  • CiNii Articles