Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells

• Yang D. Teng

  Departments of Neurology, Pediatrics, and Neurosurgery, Harvard Medical School, Boston, MA 02115; Harvard–MIT Division of Health Sciences and Technology, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; and Departments of Orthopaedic Surgery and Biostatistics, Children's Hospital, Boston, MA 02115

• Erin B. Lavik

  Departments of Neurology, Pediatrics, and Neurosurgery, Harvard Medical School, Boston, MA 02115; Harvard–MIT Division of Health Sciences and Technology, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; and Departments of Orthopaedic Surgery and Biostatistics, Children's Hospital, Boston, MA 02115

• Xianlu Qu

  Departments of Neurology, Pediatrics, and Neurosurgery, Harvard Medical School, Boston, MA 02115; Harvard–MIT Division of Health Sciences and Technology, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; and Departments of Orthopaedic Surgery and Biostatistics, Children's Hospital, Boston, MA 02115

• Kook I. Park

  Departments of Neurology, Pediatrics, and Neurosurgery, Harvard Medical School, Boston, MA 02115; Harvard–MIT Division of Health Sciences and Technology, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; and Departments of Orthopaedic Surgery and Biostatistics, Children's Hospital, Boston, MA 02115

• Jitka Ourednik

  Departments of Neurology, Pediatrics, and Neurosurgery, Harvard Medical School, Boston, MA 02115; Harvard–MIT Division of Health Sciences and Technology, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; and Departments of Orthopaedic Surgery and Biostatistics, Children's Hospital, Boston, MA 02115

• David Zurakowski

  Departments of Neurology, Pediatrics, and Neurosurgery, Harvard Medical School, Boston, MA 02115; Harvard–MIT Division of Health Sciences and Technology, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; and Departments of Orthopaedic Surgery and Biostatistics, Children's Hospital, Boston, MA 02115

• Robert Langer

  Departments of Neurology, Pediatrics, and Neurosurgery, Harvard Medical School, Boston, MA 02115; Harvard–MIT Division of Health Sciences and Technology, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; and Departments of Orthopaedic Surgery and Biostatistics, Children's Hospital, Boston, MA 02115

• Evan Y. Snyder

  Departments of Neurology, Pediatrics, and Neurosurgery, Harvard Medical School, Boston, MA 02115; Harvard–MIT Division of Health Sciences and Technology, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; and Departments of Orthopaedic Surgery and Biostatistics, Children's Hospital, Boston, MA 02115

抄録

To better direct repair following spinal cord injury (SCI), we designed an implant modeled after the intact spinal cord consisting of a multicomponent polymer scaffold seeded with neural stem cells. Implantation of the scaffold–neural stem cells unit into an adult rat hemisection model of SCI promoted long-term improvement in function (persistent for 1 year in some animals) relative to a lesion-control group. At 70 days postinjury, animals implanted with scaffold-plus-cells exhibited coordinated, weight-bearing hindlimb stepping. Histology and immunocytochemical analysis suggested that this recovery might be attributable partly to a reduction in tissue loss from secondary injury processes as well as in diminished glial scarring. Tract tracing demonstrated corticospinal tract fibers passing through the injury epicenter to the caudal cord, a phenomenon not present in untreated groups. Together with evidence of enhanced local GAP-43 expression not seen in controls, these findings suggest a possible regeneration component. These results may suggest a new approach to SCI and, more broadly, may serve as a prototype for multidisciplinary strategies against complex neurological problems.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 99 (5), 3024-3029, 2002-02-26

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360855569023824512
• NII論文ID: 80015203497
• DOI: 10.1073/pnas.052678899
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.052678899
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