Interleukin-1β Expression in Human Gastric Carcinoma with Epstein-Barr Virus Infection

• Ja-Mun Chong

  Department of Pathology, Graduate School of Medicine

• Kazuya Sakuma

  Department of Surgery

• Makoto Sudo

  Tochigi, and Department of Surgery, Yamanashi Medical UniversityYamanashi,Japan

• Toshio Osawa

  Department of Pathology, Graduate School of Medicine

• Etsuko Ohara

  Department of Pathology, Graduate School of Medicine

• Hiroshi Uozaki

  Department of Pathology, Graduate School of Medicine

• Junji Shibahara

  Department of Pathology, Graduate School of Medicine

• Kenji Kuroiwa

  Department of Applied Biological Science, Graduate School of Science and Technology

• Shin-ichi Tominaga

  Department of Biochemistry, Jichi Medical School

• Yoshitaka Hippo

  Division of Genome Science, Research Center for Advanced Science and Technology, The University of Tokyoand

• Hiroyuki Aburatani

  Division of Genome Science, Research Center for Advanced Science and Technology, The University of Tokyoand

• Nobuaki Funata

  Department of Pathology, Tokyo Metropolitan Komagome Hospital, Tokyo,

• Masashi Fukayama

  Department of Pathology, Graduate School of Medicine

抄録

The KT tumor is a transplantable strain of a human Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC), established in severe combined immunodeficiency (SCID) mice, with which the cytokine expression of EBVaGC can be investigated without interference from the infiltrating lymphocytes. As a part of a high-density oligonucleotide array (GeneChip) analysis of EBVaGC, the interleukin-1β (IL-1β) gene was the only cytokine gene that showed markedly higher expression in the KT tumor cells than in two tumor strains of EBV-negative GC. The results were confirmed by Northern blotting, Western blotting, and enzyme-linked immunosorbent assay. Furthermore, we demonstrated a positive signal for IL-1β mRNA in the carcinoma cells of a surgically resected EBVaGC, but not in EBV-negative GC, by in situ hybridization. In vitro, IL-1β increased the cell growth of a GC cell line, TMK1. Thus, IL-1β may act as an autocrine growth factor in EBVaGC.

収録刊行物

• Journal of Virology

  Journal of Virology 76 (13), 6825-6831, 2002-07

  American Society for Microbiology

キーワード

• Virology
• Insect Science
• Immunology
• Microbiology

詳細情報

• CRID: 1360292619339027456
• NII論文ID: 80015380548
• DOI: 10.1128/jvi.76.13.6825-6831.2002
• ISSN: 10985514
• ISSN: 0022538X
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JVI.76.13.6825-6831.2002
• データソース種別:
  • Crossref
  • CiNii Articles