Whole-Genome Shotgun Assembly and Analysis of the Genome of <i>Fugu rubripes</i>
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- Samuel Aparicio
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Jarrod Chapman
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Elia Stupka
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Nik Putnam
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Jer-ming Chia
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Paramvir Dehal
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Alan Christoffels
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Sam Rash
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Shawn Hoon
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Arian Smit
- Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.
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- Maarten D. Sollewijn Gelpke
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Jared Roach
- Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.
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- Tania Oh
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Isaac Y. Ho
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Marie Wong
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Chris Detter
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Frans Verhoef
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Paul Predki
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Alice Tay
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Susan Lucas
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Paul Richardson
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Sarah F. Smith
- MRC UK HGMP Resource Centre, Hinxton, Cambridge CB10 1SB, UK.
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- Melody S. Clark
- MRC UK HGMP Resource Centre, Hinxton, Cambridge CB10 1SB, UK.
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- Yvonne J. K. Edwards
- MRC UK HGMP Resource Centre, Hinxton, Cambridge CB10 1SB, UK.
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- Norman Doggett
- Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
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- Andrey Zharkikh
- Myriad Genetics Inc., 320 Wakara Way, Salt Lake City, UT 84108, USA.
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- Sean V. Tavtigian
- Myriad Genetics Inc., 320 Wakara Way, Salt Lake City, UT 84108, USA.
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- Dmitry Pruss
- Myriad Genetics Inc., 320 Wakara Way, Salt Lake City, UT 84108, USA.
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- Mary Barnstead
- Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.
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- Cheryl Evans
- Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.
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- Holly Baden
- Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.
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- Justin Powell
- Paradigm Therapeutics Ltd., Physiological Laboratory, Cambridge CB2 3EG, UK.
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- Gustavo Glusman
- Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.
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- Lee Rowen
- Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.
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- Leroy Hood
- Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.
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- Y. H. Tan
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Greg Elgar
- MRC UK HGMP Resource Centre, Hinxton, Cambridge CB10 1SB, UK.
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- Trevor Hawkins
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Byrappa Venkatesh
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
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- Daniel Rokhsar
- U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
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- Sydney Brenner
- Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.
抄録
<jats:p> The compact genome of <jats:italic>Fugu rubripes</jats:italic> has been sequenced to over 95% coverage, and more than 80% of the assembly is in multigene-sized scaffolds. In this 365-megabase vertebrate genome, repetitive DNA accounts for less than one-sixth of the sequence, and gene loci occupy about one-third of the genome. As with the human genome, gene loci are not evenly distributed, but are clustered into sparse and dense regions. Some “giant” genes were observed that had average coding sequence sizes but were spread over genomic lengths significantly larger than those of their human orthologs. Although three-quarters of predicted human proteins have a strong match to <jats:italic>Fugu</jats:italic> , approximately a quarter of the human proteins had highly diverged from or had no pufferfish homologs, highlighting the extent of protein evolution in the 450 million years since teleosts and mammals diverged. Conserved linkages between <jats:italic>Fugu</jats:italic> and human genes indicate the preservation of chromosomal segments from the common vertebrate ancestor, but with considerable scrambling of gene order. </jats:p>
収録刊行物
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- Science
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Science 297 (5585), 1301-1310, 2002-08-23
American Association for the Advancement of Science (AAAS)
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詳細情報 詳細情報について
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- CRID
- 1363388845291002752
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- NII論文ID
- 80015480139
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- ISSN
- 10959203
- 00368075
- http://id.crossref.org/issn/00368075
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