Visualization by comprehensive microarray analysis of gene expression programs during transdifferentiation of mesophyll cells into xylem cells
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- Taku Demura
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Gen Tashiro
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Gorou Horiguchi
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Naoki Kishimoto
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Minoru Kubo
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Naoko Matsuoka
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Atsushi Minami
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Miyo Nagata-Hiwatashi
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Keiko Nakamura
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Yoshimichi Okamura
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Naomi Sassa
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Shinsuke Suzuki
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Junshi Yazaki
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Shoshi Kikuchi
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
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- Hiroo Fukuda
- Plant Science Center, RIKEN, 230-0045 Yokohama, Japan; Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 113-0033 Tokyo, Japan; Department of Material Engineering, Tsuruoka National College of Technology, 997-8511 Tsuruoka, Japan; Food Research Center, Aichi Industrial Technology Institute, 451-0083 Nagoya, Japan; and Institute of the Society for Techno...
抄録
<jats:p> Plants have a unique transdifferentiation mechanism by which differentiated cells can initiate a new program of differentiation. We used a comprehensive analysis of gene expression in an <jats:italic>in vitro</jats:italic> zinnia ( <jats:italic>Zinnia elegans</jats:italic> L.) culture model system to gather fundamental information about the gene regulation underlying the transdifferentiation of plant cells. In this model, photosynthetic mesophyll cells isolated from zinnia leaves transdifferentiate into xylem cells in a morphogenic process characterized by features such as secondary-wall formation and programmed cell death. More than 8,000 zinnia cDNA clones were isolated from an equalized cDNA library prepared from cultured cells transdifferentiating into xylem cells. Microarray analysis using these cDNAs revealed several types of unique gene regulation patterns, including: the transient expression of a set of genes during cell isolation, presumably induced by wounding; a rapid reduction in the expression of photosynthetic genes and the rapid induction of protein synthesis-associated genes during the first stage; the preferential induction of auxin-related genes during the subsequent stage; and the transient induction of genes closely associated with particular morphogenetic events, including cell-wall formation and degradation and programmed cell death during the final stage. This analysis also revealed a number of previously uncharacterized genes encoding proteins that function in signal transduction, such as protein kinases and transcription factors that are expressed in a stage-specific manner. These findings provide new clues to the molecular mechanisms of both plant transdifferentiation and wood formation. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 99 (24), 15794-15799, 2002-11-18
Proceedings of the National Academy of Sciences