Identification and functional expression of a second human β‐galactoside α2,6‐sialyltransferase, ST6Gal II

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<jats:p> <jats:sc>blast</jats:sc> analysis of the human and mouse genome sequence databases using the sequence of the human CMP‐sialic acid:β‐galactoside α‐2,6‐sialyltransferase cDNA (hST6Gal I, EC2.4.99.1) as a probe allowed us to identify a putative sialyltransferase gene on chromosome 2. The sequence of the corresponding cDNA was also found as an expressed sequence tag of human brain. This gene contained a 1590 bp open reading frame divided in five exons and the deduced amino‐acid sequence didn't correspond to any sialyltransferase already known in other species. Multiple sequence alignment and subsequent phylogenic analysis showed that this new enzyme belonged to the ST6Gal subfamily and shared 48% identity with hST6Gal‐I. Consequently, we named this new sialyltransferase ST6Gal II. A construction in pFlag vector transfected in COS‐7 cells gave raise to a soluble active form of ST6Gal II. Enzymatic assays indicate that the best acceptor substrate of ST6Gal II was the free disaccharide Galβ1–4GlcNAc structure whereas ST6Gal I preferred Galβ1–4GlcNAc‐R disaccharide sequence linked to a protein. The α2,6‐linkage was confirmed by the increase of <jats:italic>Sambucus nigra</jats:italic> agglutinin‐lectin binding to the cell surface of CHO transfected with the cDNA encoding ST6Gal II and by specific sialidases treatment. In addition, the ST6Gal II gene showed a very tissue specific pattern of expression because it was found essentially in brain whereas ST6Gal I gene is ubiquitously expressed.</jats:p>

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