Comprehensive Studies of Drug Resistance Mediated by Overexpression of Response Regulators of Two-Component Signal Transduction Systems in <i>Escherichia coli</i>

  • Hidetada Hirakawa
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki
  • Kunihiko Nishino
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki
  • Takahiro Hirata
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki
  • Akihito Yamaguchi
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki

抄録

<jats:title>ABSTRACT</jats:title> <jats:p> In <jats:italic>Escherichia coli</jats:italic> , there are 32 open reading frames (ORFs) that are assumed to be response regulator genes of two-component signal transduction systems on the basis of sequence similarities. We cloned all of these 32 ORFs into a multicopy expression vector and investigated whether or not they confer drug resistance via control of drug resistance determinants. Fifteen of these ORFs, i.e., <jats:italic>baeR</jats:italic> , <jats:italic>citB</jats:italic> , <jats:italic>cpxR</jats:italic> , <jats:italic>evgA</jats:italic> , <jats:italic>fimZ</jats:italic> , <jats:italic>kdpE</jats:italic> , <jats:italic>narL</jats:italic> , <jats:italic>narP</jats:italic> , <jats:italic>ompR</jats:italic> , <jats:italic>rcsB</jats:italic> , <jats:italic>rstA</jats:italic> , <jats:italic>torR</jats:italic> , <jats:italic>yedW</jats:italic> , <jats:italic>yehT</jats:italic> , and <jats:italic>dcuR</jats:italic> , conferred increased single- or multidrug resistance. Two-thirds of them conferred deoxycholate resistance. Five of them, i.e., <jats:italic>evgA</jats:italic> , <jats:italic>baeR</jats:italic> , <jats:italic>ompR, cpxR</jats:italic> , and <jats:italic>rcsB</jats:italic> , modulated the expression of several drug exporter genes. The drug resistance mediated by <jats:italic>evgA</jats:italic> , <jats:italic>baeR</jats:italic> , and <jats:italic>cpxR</jats:italic> could be assigned to drug exporters by using drug exporter gene knockout strains. </jats:p>

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