New PCR for rapid identification of Mycoplasma pneumoniae in clinical specimens from children with respiratory tract infections
-
- Morozumi Miyuki
- Graduate School of Infection Control Sciences, Kitasato University
-
- Iwata Satoshi
- National Tokyo Medical Center
-
- Endo Hiroko
- Tohoku Rosai Hospital
-
- Oishi Tomohiro
- Department of Pediatrics, Joetsu General Hospital
-
- Ohnari Shigeru
- Nakafukawa Pediatric Clinic
-
- Kawamura Naohisa
- Osaka Rosai Hospital Department of Pediatrics, Osaka Medical College
-
- Kuroki Haruo
- Department of Pediatrics, Nagatsu-kai Saitoh Hospital
-
- Kobayashi Masaaki
- Kobayashi Pediatric Clinic
-
- Saito Kouta
- Saito Pediatric Clinic
-
- Sakai Ritsuko
- Sakai Clinic
-
- Sunakawa Keisuke
- Department of Infectious Diseases, Kitasato University School of Medicine
-
- Tajima Takeshi
- Hakujikai Memorial Hospital
-
- Nitta Masahiko
- Department of Pediatrics, Seikeikai Hospital Department of Pediatrics, Osaka Medical College
-
- Nonoyama Masato
- Department of Infectious Diseases, Kitasato University School of Medicine
-
- Kobayashi Reiko
- Pharmaceutical Reseach Center, Meiji Seika Kaisha, Ltd.
-
- Chiba Naoko
- Graduate School of Infection Control Sciences, Kitasato University
-
- Ubukata Kimiko
- Laboratory of Infectious Agents Surveillance, Kitasato Institute for Life Sciences, Kitasato University
Bibliographic Information
- Other Title
-
- Mycoplasma pneumoniaeの迅速検索を目的としたPCR―小児呼吸器感染症検体を用いて―
- specimens from children with respiratory tract infections
- 小児呼吸器感染症検体を用いて
Search this article
Abstract
We designed a set of primers on the 16 S rRNA gene to directly detect Mycoplasma pneumoniae by PCR in clinical specimens from patients with respiratory infections. The sensitivity of the primers was 2 CFU/tube under the following conditions: 35 cycles of 15 sec at 94°C, 15 sec at 53°C and 15 sec at 72°C /cycle. We therefore judged the PCR results to be positive if the CFU for M. pneumoniae in a clinical specimen was 1.1×103. The results of the PCR were obtained in about 2.5 h. We then used the PCR to examine 783 clinical specimens (epipharynx [n=612], throat [n=141], ear discharge [n=12] etc.) collected from pediatric patients between May 2002 and January 2003. The PCR results were positive in 79 cases (10.1%); 58/291 cases (19.9%) corresponded to specimens from patients with pneumonia, 13/207 cases (6.3%) to specimens from patients with acute bronchitis, 2/130 cases (1.5%) to those from patients with acute pharyngitis and 2/45 cases (4.4%) to those from patients with acute upper respiratory infections. The percentage of PCRpositive cases was significantly higher among the patients with lower part respiratory tract infections (x2=53.3008, p=0.0000). Among the patients with pneumonia, 65 were diagnosed with M. pneumoniae based on a significant rise or high antibody titer for M. pneumoniae as determined by the PA or CF test, and 46 of these cases were PCR-positive (70.8%). We also investigated the correlation between the antibiotics used before PCR and the PCR results in these 65 cases. Of the total of 43 cases, 21 not treated with antibiotics before PCR and 22 cases given oral β-lactam agents ineffective against M. pneumoniae, 38 (88.4%) were PCR-positive, whereas only 3 (23.1%) of the specimens from 13 patients treated with macrolides or new quinolones effective against M. pneumoniae were PCR-positive. We concluded from these results, that the PCR is very useful in selecting suitable chemotherapeutic agents for patients not treated with antibiotics, however it may be necessary to consider the influence of days after to develop infection on PCR results.
Journal
-
- Japanese Journal of Chemotherapy
-
Japanese Journal of Chemotherapy 51 (5), 289-299, 2003
Japanese Society of Chemotherapy
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001206284928000
-
- NII Article ID
- 130004298167
- 80015958816
-
- NII Book ID
- AN10472127
-
- ISSN
- 18845886
- 13407007
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed