Flow-through Micro Sensor Using Immobilized Peroxidase with Chemiluminometric FIA System for Determining Hydrogen Peroxide

• KIBA Nobutoshi

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• TOKIZAWA Toyonari

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• KATO Sayaka

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• TACHIBANA Masaki

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• TANI Kazue

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• KOIZUMI Hitoshi

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• EDO Masaharu

  Functional Divice Laboratory, Fuji Electric Corporate Research and Development

• YONEZAWA Eiichi

  Functional Divice Laboratory, Fuji Electric Corporate Research and Development

抄録

A micromachined flow cell (overall size; 25 × 25 × 1 mm³) was designed for the fast determination of hydrogen peroxide, based on a luminol-H₂O₂ chemiluminescence reaction catalyzed by immobilized peroxidase (POD). The flow cell consisted of a sandwich of anisotropically etched silicon and glass chips and contained a spiral channel (20 turns, 50 cm long, 150 μm wide, 20 μm depth, channel volume 1.4 μl) and two holes (1 mm diameter). POD was covalently immobilized with 3-(trimethoxysilyl)propyldietylenetriamine and glutaraldehyde on the inner surface of the channel. The chip was placed in front of a window of a photomultiplier tube and used as a flow cell in a single-line flow-injection analysis system using a luminol solution as a carrier solution. The sample volume for one measurement was 0.2 μl. The maximal sampling rate was 315 h^-1 at a carrier solution flow rate of 10 μl min^-1. A calibration graph for H₂O₂ was linear for 5 nM - 5 μM; the detection limit (signal-to-noise = 3) was 1 nM (7 fg in 0.2 μl injection). The H₂O₂ concentration in rainwater was determined using this sensor system.

収録刊行物

• Analytical Sciences

  Analytical Sciences 19 (6), 823-827, 2003

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390282679232583680
• NII論文ID: 130004826097
• NII論文ID: 80015993343
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.19.823
• COI: 1:CAS:528:DC%2BD3sXkslOkurg%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 6560599
• PubMed: 12834219
• Web Site: http://id.ndl.go.jp/bib/6560599
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6560599
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.19.823.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.19.823/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/19/6/19_6_823/_pdf
• 本文言語コード: en
• データソース種別:
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