Application of 4-(4-Nitrophenyl)- 1,2,4-triazoline-3,5-dione to Analysis of 25-Hydroxyvitamin D3 in Human Plasma by Liquid Chromatography/Electron Capture Atmospheric Pressure Chemical Ionization-Mass Spectrometry

• HIGASHI Tatsuya

  Faculty of Pharmaceutical Sciences, Kanazawa University

• YAMAUCHI Akinori

  Faculty of Pharmaceutical Sciences, Kanazawa University

• SHIMADA Kazutake

  Faculty of Pharmaceutical Sciences, Kanazawa University

Abstract

The utility of 4-(4-nitrophenyl)-1,2,4-triazoline-3,5-dione (NPTAD) as a derivatization reagent in the analysis of 25-hydroxyvitamin D₃ [25(OH)D₃] using liquid chromatography/electron capture atmospheric pressure chemical ionization-mass spectrometry (LC/ECAPCI-MS) was examined. The derivatives of 25(OH)D₃ with NPTAD underwent electron capture in the APCI source in the negative-ion mode and provided 30-fold higher sensitivity compared to an intact compound. This derivatization-LC/ECAPCI-MS method was applied to a plasma 25-hydroxyvitamin D₃ assay, and gave satisfactory results in sensitivity, specificity, measurable range and throughput of the analysis.

Journal

• Analytical Sciences

  Analytical Sciences 19 (6), 941-943, 2003

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390282679232565504
• NII Article ID: 130004826118
• NII Article ID: 80015993364
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.19.941
• COI: 1:CAS:528:DC%2BD3sXkslOrsrk%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 6560922
• PubMed: 12834240
• Web Site: http://hdl.handle.net/2297/29324
• Web Site: http://id.ndl.go.jp/bib/6560922
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6560922
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.19.941.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.19.941/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/19/6/19_6_941/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2004082001
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed