Mechanisms of Glucose Signaling during Germination of Arabidopsis

DOI PDF 被引用文献7件
  • John Price
    Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio 43210
  • Tsai-Chi Li
    Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio 43210
  • Shin Gene Kang
    Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio 43210
  • Jong Kuk Na
    Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio 43210
  • Jyan-Chyun Jang
    Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio 43210

抄録

<jats:title>Abstract</jats:title><jats:p>Glucose (Glc) signaling, along with abscisic acid (ABA) signaling, has been implicated in regulating early plant development in Arabidopsis. It is generally believed that high levels of exogenous Glc cause ABA accumulation, which results in a delay of germination and an inhibition of seedling development—a typical stress response. To test this hypothesis and decipher the complex interactions that occur in the signaling pathways, we determined the effects of sugar and ABA on one developmental event, germination. We show that levels of exogenous Glc lower than previously cited could delay the rate of seed germination in wild-ecotype seeds. Remarkably, this effect could not be mimicked by an osmotic effect, and ABA was still involved. With higher concentrations of Glc, previously known Glc-insensitive mutants gin2 and abi4 exhibited germination kinetics similar to wild type, indicating that Glc-insensitive phenotypes are not the same for all developmental stages of growth and that the signaling properties of Glc vary with concentration. Higher concentrations of Glc were more potent in delaying seed germination. However, Glc-delayed seed germination was not caused by increased cellular ABA concentration, rather Glc appeared to slow down the decline of endogenous ABA. Except for the ABA-insensitive mutants, all tested genotypes appeared to have similar ABA perception during germination, where germination was correlated with the timing of ABA drop to a threshold level. In addition, Glc was found to modulate the transcription of genes involved in ABA biosynthesis and perception only after germination, suggesting a critical role of the developmental program in sugar sensing. On the basis of an extensive phenotypic, biochemical, and molecular analysis, we suggest that exogenous Glc application creates specific signals that vary with concentration and the developmental stage of the plant and that Glc-induced fluctuations in endogenous ABA level generate a different set of signals than those generated by external ABA application.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 132 (3), 1424-1438, 2003-07-01

    Oxford University Press (OUP)

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