Negative Feedback Regulation Ensures the One Receptor-One Olfactory Neuron Rule in Mouse

Shou Serizawa, Kazunari Miyamichi, Hiroko Nakatani, Misao Suzuki, Michiko Saito, Yoshihiro Yoshihara, Hitoshi Sakano

doi:10.1126/science.1089122

Negative Feedback Regulation Ensures the One Receptor-One Olfactory Neuron Rule in Mouse

DOI Web Site 被引用文献40件

Shou Serizawa

Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0032, Japan.
Kazunari Miyamichi

Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0032, Japan.
Hiroko Nakatani

Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0032, Japan.
Misao Suzuki

Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0032, Japan.
Michiko Saito

Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0032, Japan.
Yoshihiro Yoshihara

Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0032, Japan.
Hitoshi Sakano

Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0032, Japan.

抄録

<jats:p>In the mouse olfactory system, each olfactory sensory neuron (OSN) expresses only one odorant receptor (OR) gene in a monoallelic and mutually exclusive manner. Such expression forms the genetic basis for OR-instructed axonal projection of OSNs to the olfactory bulb of the brain during development. Here, we identify an upstream cis-acting DNA region that activates the OR gene cluster in mouse and allows the expression of only one OR gene within the cluster. Deletion of the coding region of the expressed OR gene or a naturally occurring frame-shift mutation allows a second OR gene to be expressed. We propose that stochastic activation of only one OR gene within the cluster and negative feedback regulation by that OR gene product are necessary to ensure the one receptor–one neuron rule.</jats:p>