Species-Specific Recognition of Single-Stranded RNA via Toll-like Receptor 7 and 8

• Florian Heil

  Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

• Hiroaki Hemmi

  Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

• Hubertus Hochrein

  Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

• Franziska Ampenberger

  Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

• Carsten Kirschning

  Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

• Shizuo Akira

  Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

• Grayson Lipford

  Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

• Hermann Wagner

  Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

• Stefan Bauer

  Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

抄録

Double-stranded ribonucleic acid (dsRNA) serves as a danger signal associated with viral infection and leads to stimulation of innate immune cells. In contrast, the immunostimulatory potential of single-stranded RNA (ssRNA) is poorly understood and innate immune receptors for ssRNA are unknown. We report that guanosine (G)- and uridine (U)-rich ssRNA oligonucleotides derived from human immunodeficiency virus–1 (HIV-1) stimulate dendritic cells (DC) and macrophages to secrete interferon-α and proinflammatory, as well as regulatory, cytokines. By using Toll-like receptor (TLR)–deficient mice and genetic complementation, we show that murine TLR7 and human TLR8 mediate species-specific recognition of GU-rich ssRNA. These data suggest that ssRNA represents a physiological ligand for TLR7 and TLR8.

収録刊行物

• Science

  Science 303 (5663), 1526-1529, 2004-03-05

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361137044400995328
• NII論文ID: 80016545722
• DOI: 10.1126/science.1093620
• ISSN: 10959203
• ISSN: 00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.1093620
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