Aerobic and Anaerobic Toluene Degradation by a Newly Isolated Denitrifying Bacterium, <i>Thauera</i> sp. Strain DNT-1

DOI Web Site 被引用文献5件
  • Yoshifumi Shinoda
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502
  • Yasuyoshi Sakai
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502
  • Hiroshi Uenishi
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502
  • Yasumitsu Uchihashi
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502
  • Akira Hiraishi
    Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi 441-8580
  • Hideaki Yukawa
    Research Institute of Innovative Technology for the Earth, Soraku-gun, Kyoto 619-0292, Japan
  • Hiroya Yurimoto
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502
  • Nobuo Kato
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502

抄録

<jats:title>ABSTRACT</jats:title> <jats:p> A newly isolated denitrifying bacterium, <jats:italic>Thauera</jats:italic> sp. strain DNT-1, grew on toluene as the sole carbon and energy source under both aerobic and anaerobic conditions. When this strain was cultivated under oxygen-limiting conditions with nitrate, first toluene was degraded as oxygen was consumed, while later toluene was degraded as nitrate was reduced. Biochemical observations indicated that initial degradation of toluene occurred through a dioxygenase-mediated pathway and the benzylsuccinate pathway under aerobic and denitrifying conditions, respectively. Homologous genes for toluene dioxygenase ( <jats:italic>tod</jats:italic> ) and benzylsuccinate synthase ( <jats:italic>bss</jats:italic> ), which are the key enzymes in aerobic and anaerobic toluene degradation, respectively, were cloned from genomic DNA of strain DNT-1. The results of Northern blot analyses and real-time quantitative reverse transcriptase PCR suggested that transcription of both sets of genes was induced by toluene. In addition, the <jats:italic>tod</jats:italic> genes were induced under aerobic conditions, whereas the <jats:italic>bss</jats:italic> genes were induced under both aerobic and anaerobic conditions. On the basis of these results, it is concluded that strain DNT-1 modulates the expression of two different initial pathways of toluene degradation according to the availability of oxygen in the environment. </jats:p>

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