Arsenite-Oxidizing <i>Hydrogenobaculum</i> Strain Isolated from an Acid-Sulfate-Chloride Geothermal Spring in Yellowstone National Park

  • Jessica Donahoe-Christiansen
    Thermal Biology Institute and Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, Montana 59717
  • Seth D'Imperio
    Thermal Biology Institute and Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, Montana 59717
  • Colin R. Jackson
    Thermal Biology Institute and Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, Montana 59717
  • William P. Inskeep
    Thermal Biology Institute and Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, Montana 59717

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<jats:title>ABSTRACT</jats:title> <jats:p> An arsenite-oxidizing <jats:italic>Hydrogenobaculum</jats:italic> strain was isolated from a geothermal spring in Yellowstone National Park, Wyo., that was previously shown to contain microbial populations engaged in arsenite oxidation. The isolate was sensitive to both arsenite and arsenate and behaved as an obligate chemolithoautotroph that used H <jats:sub>2</jats:sub> as its sole energy source and had an optimum temperature of 55 to 60°C and an optimum pH of 3.0. The arsenite oxidation in this organism displayed saturation kinetics and was strongly inhibited by H <jats:sub>2</jats:sub> S. </jats:p>

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