<i>Bacillus subtilis</i>SalA (YbaL) Negatively Regulates Expression of<i>scoC</i>, Which Encodes the Repressor for the Alkaline Exoprotease Gene,<i>aprE</i>
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- Mitsuo Ogura
- Department of Marine Science, School of Marine Science and Technology, Tokai University, Shizuoka 424-8610
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- Atsushi Matsuzawa
- Department of Marine Science, School of Marine Science and Technology, Tokai University, Shizuoka 424-8610
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- Hirofumi Yoshikawa
- Department of Biosciences, Tokyo University of Agriculture, Setagaya-Ku, Sakuraoka, Tokyo 156-8502, Japan
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- Teruo Tanaka
- Department of Marine Science, School of Marine Science and Technology, Tokai University, Shizuoka 424-8610
抄録
<jats:title>ABSTRACT</jats:title><jats:p>During the course of screening for exoprotease-deficient mutants among<jats:italic>Bacillus subtilis</jats:italic>gene disruptants, a strain showing such a phenotype was identified. The locus responsible for this phenotype was the previously unknown gene<jats:italic>ybaL</jats:italic>, which we renamed<jats:italic>salA.</jats:italic>The predicted gene product encoded by<jats:italic>salA</jats:italic>belongs to the Mrp family, which is widely conserved among archaea, prokaryotes, and eukaryotes. Disruption of<jats:italic>salA</jats:italic>resulted in a decrease in the expression of a<jats:italic>lacZ</jats:italic>fusion of the<jats:italic>aprE</jats:italic>gene encoding the major extracellular alkaline protease. The decrease was recovered by the cloned<jats:italic>salA</jats:italic>gene on a plasmid, demonstrating that the gene is involved in<jats:italic>aprE</jats:italic>expression. Determination of the<jats:italic>cis</jats:italic>-acting region of SalA on the upstream region of<jats:italic>aprE</jats:italic>, together with epistatic analyses with<jats:italic>scoC</jats:italic>,<jats:italic>abrB</jats:italic>, and<jats:italic>spo0A</jats:italic>mutations that also affect<jats:italic>aprE</jats:italic>expression, suggested that<jats:italic>salA</jats:italic>deficiency affects<jats:italic>aprE-lacZ</jats:italic>expression through the negative regulator ScoC. Northern and reverse transcription-PCR analyses revealed enhanced levels of<jats:italic>scoC</jats:italic>transcripts in the<jats:italic>salA</jats:italic>mutant cells in the transition and early stationary phases. Concomitant with these observations, larger amounts of the ScoC protein were detected in the mutant cells by Western analysis. From these results we conclude that SalA negatively regulates<jats:italic>scoC</jats:italic>expression. It was also found that the expression of a<jats:italic>salA-lacZ</jats:italic>fusion was increased by<jats:italic>salA</jats:italic>deficiency, suggesting that<jats:italic>salA</jats:italic>is autoregulated.</jats:p>
収録刊行物
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- Journal of Bacteriology
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Journal of Bacteriology 186 (10), 3056-3064, 2004-05-15
American Society for Microbiology
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詳細情報 詳細情報について
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- CRID
- 1361699994730608640
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- NII論文ID
- 80016745616
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- ISSN
- 10985530
- 00219193
- http://id.crossref.org/issn/00219193
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