Novel Regulation of Aquaporins during Osmotic Stress

• Rosario Vera-Estrella

  Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Cuernavaca, Morelos, 62250, Mexico (R.V.-E., B.J.B., O.P.); and Departments of Plant Biology and Crop Sciences, University of Illinois, Urbana, Illinois 61801–3838 (H.J.B.)

• Bronwyn J. Barkla

  Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Cuernavaca, Morelos, 62250, Mexico (R.V.-E., B.J.B., O.P.); and Departments of Plant Biology and Crop Sciences, University of Illinois, Urbana, Illinois 61801–3838 (H.J.B.)

• Hans J. Bohnert

  Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Cuernavaca, Morelos, 62250, Mexico (R.V.-E., B.J.B., O.P.); and Departments of Plant Biology and Crop Sciences, University of Illinois, Urbana, Illinois 61801–3838 (H.J.B.)

• Omar Pantoja

  Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Cuernavaca, Morelos, 62250, Mexico (R.V.-E., B.J.B., O.P.); and Departments of Plant Biology and Crop Sciences, University of Illinois, Urbana, Illinois 61801–3838 (H.J.B.)

抄録

Aquaporin protein regulation and redistribution in response to osmotic stress was investigated. Ice plant (Mesembryanthemum crystallinum) McTIP1;2 (McMIPF) mediated water flux when expressed in Xenopus leavis oocytes. Mannitol-induced water imbalance resulted in increased protein amounts in tonoplast fractions and a shift in protein distribution to other membrane fractions, suggesting aquaporin relocalization. Indirect immunofluorescence labeling also supports a change in membrane distribution for McTIP1;2 and the appearance of a unique compartment where McTIP1;2 is expressed. Mannitol-induced redistribution of McTIP1;2 was arrested by pretreatment with brefeldin A, wortmannin, and cytochalasin D, inhibitors of vesicle trafficking-related processes. Evidence suggests a role for glycosylation and involvement of a cAMP-dependent signaling pathway in McTIP1;2 redistribution. McTIP1;2 redistribution to endosomal compartments may be part of a homeostatic process to restore and maintain cellular osmolarity under osmotic-stress conditions.

収録刊行物

• Plant Physiology

  Plant Physiology 135 (4), 2318-2329, 2004-08-01

  Oxford University Press (OUP)

キーワード

• Plant Science
• Genetics
• Physiology

詳細情報

• CRID: 1361981470446944384
• NII論文ID: 80016925239
• DOI: 10.1104/pp.104.044891
• ISSN: 15322548
• ISSN: 00320889
• Web Site: http://academic.oup.com/plphys/article-pdf/135/4/2318/38704303/plphys_v135_4_2318.pdf
• データソース種別:
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  • CiNii Articles