Hyper-inducible expression system for streptomycetes

• Sachio Herai

  Institute of Applied Biochemistry and Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan; and The Kitasato Institute, Tokyo 108-8642, Japan

• Yoshiteru Hashimoto

  Institute of Applied Biochemistry and Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan; and The Kitasato Institute, Tokyo 108-8642, Japan

• Hiroki Higashibata

  Institute of Applied Biochemistry and Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan; and The Kitasato Institute, Tokyo 108-8642, Japan

• Hideaki Maseda

  Institute of Applied Biochemistry and Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan; and The Kitasato Institute, Tokyo 108-8642, Japan

• Haruo Ikeda

  Institute of Applied Biochemistry and Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan; and The Kitasato Institute, Tokyo 108-8642, Japan

• Satoshi Ōmura

  Institute of Applied Biochemistry and Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan; and The Kitasato Institute, Tokyo 108-8642, Japan

• Michihiko Kobayashi

  Institute of Applied Biochemistry and Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan; and The Kitasato Institute, Tokyo 108-8642, Japan

抄録

Streptomycetes produce useful enzymes and a wide variety of secondary metabolites with potent biological activities (e.g., antibiotics, immunosuppressors, pesticides, etc.). Despite their importance in the pharmaceutical and agrochemical fields, there have been no reports for practical expression systems in streptomycetes. Here, we developed a “P nitA -NitR” system for regulatory gene expression in streptomycetes based on the expression mechanism of Rhodococcus rhodochrous J1 nitrilase, which is highly induced by an inexpensive and safe inducer, ε-caprolactam. Heterologous protein expression experiments demonstrated that the system allowed suppressed basal expression and hyper-inducible expression, yielding target protein levels of as high as ≈40% of all soluble protein. Furthermore, the system functioned in important streptomycete strains. Thus, the P nitA -NitR system should be a powerful tool for improving the productivity of various useful products in streptomycetes.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 101 (39), 14031-14035, 2004-09-17

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360855568853278720
• NII論文ID: 80017030795
• DOI: 10.1073/pnas.0406058101
• ISSN: 10916490
• ISSN: 00278424
• ISSN: http://id.crossref.org/issn/00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.0406058101
• データソース種別:
  • Crossref
  • CiNii Articles