Roles of Four Chitinases (ChiA, ChiB, ChiC, and ChiD) in the Chitin Degradation System of Marine Bacterium <i>Alteromonas</i> sp. Strain O-7

• Hideyuki Orikoshi

  Department of Microbiology, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan

• Shigenari Nakayama

  Department of Microbiology, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan

• Katsushiro Miyamoto

  Department of Microbiology, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan

• Chiaki Hanato

  Department of Microbiology, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan

• Masahide Yasuda

  Department of Microbiology, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan

• Yoshihiko Inamori

  Department of Microbiology, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan

• Hiroshi Tsujibo

  Department of Microbiology, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan

抄録

Alteromonas sp. strain O-7 secretes four chitinases (ChiA, ChiB, ChiC, and ChiD) in the presence of chitin. To elucidate why the strain produces multiple chitinases, we studied the expression levels of the four genes and proteins, their enzymatic properties, and their synergistic effects on chitin degradation. Among the four chitinases, ChiA was produced in the largest quantities, followed by ChiD, and the production of ChiB and ChiC changed at lower levels than those of ChiA and ChiD. The expression of the chiA , chiB , chiC , and chiD genes was investigated at the transcriptional level. The RNA transcript of chiA was most strongly induced in the presence of chitin, the expression of chiD followed, and the RNA transcripts of chiB and chiC changed at low levels. The hydrolyzing activities of the four chitinases against various substrates were examined. ChiA was the most active enzyme against powdered chitin, whereas ChiC was the most active against soluble chitin among the four chitinases. ChiD had activities closer to those of ChiA than to those of ChiB and ChiC. ChiB showed no distinctive feature against the chitinous substrates tested. When powdered chitin was treated with the proper combination of four chitinases, an approximately 2.0-fold increase in the hydrolytic activity was observed. These results, together with the results described above, indicate that ChiA plays a central role in chitin degradation for this strain.

収録刊行物

• Applied and Environmental Microbiology

  Applied and Environmental Microbiology 71 (4), 1811-1815, 2005-04

  American Society for Microbiology

キーワード

• Ecology
• Applied Microbiology and Biotechnology
• Food Science
• Biotechnology

詳細情報

• CRID: 1364233270556221440
• NII論文ID: 80017369191
• DOI: 10.1128/aem.71.4.1811-1815.2005
• ISSN: 10985336
• ISSN: 00992240
• Web Site: https://journals.asm.org/doi/pdf/10.1128/AEM.71.4.1811-1815.2005
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