Cloning of a human parvovirus by molecular screening of respiratory tract samples

DOI Web Site 被引用文献36件
  • Tobias Allander
    Department of Clinical Microbiology and Center for Molecular Medicine, Karolinska University Hospital, Department of Woman and Child Health, Astrid Lindgren Children's Hospital, and Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; and Departments of Biological Sciences and Biochemistry, National University of Singapore, 14 Science Drive 4, Singapore 117 543, Singapore
  • Martti T. Tammi
    Department of Clinical Microbiology and Center for Molecular Medicine, Karolinska University Hospital, Department of Woman and Child Health, Astrid Lindgren Children's Hospital, and Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; and Departments of Biological Sciences and Biochemistry, National University of Singapore, 14 Science Drive 4, Singapore 117 543, Singapore
  • Margareta Eriksson
    Department of Clinical Microbiology and Center for Molecular Medicine, Karolinska University Hospital, Department of Woman and Child Health, Astrid Lindgren Children's Hospital, and Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; and Departments of Biological Sciences and Biochemistry, National University of Singapore, 14 Science Drive 4, Singapore 117 543, Singapore
  • Annelie Bjerkner
    Department of Clinical Microbiology and Center for Molecular Medicine, Karolinska University Hospital, Department of Woman and Child Health, Astrid Lindgren Children's Hospital, and Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; and Departments of Biological Sciences and Biochemistry, National University of Singapore, 14 Science Drive 4, Singapore 117 543, Singapore
  • Annika Tiveljung-Lindell
    Department of Clinical Microbiology and Center for Molecular Medicine, Karolinska University Hospital, Department of Woman and Child Health, Astrid Lindgren Children's Hospital, and Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; and Departments of Biological Sciences and Biochemistry, National University of Singapore, 14 Science Drive 4, Singapore 117 543, Singapore
  • Björn Andersson
    Department of Clinical Microbiology and Center for Molecular Medicine, Karolinska University Hospital, Department of Woman and Child Health, Astrid Lindgren Children's Hospital, and Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; and Departments of Biological Sciences and Biochemistry, National University of Singapore, 14 Science Drive 4, Singapore 117 543, Singapore

抄録

<jats:p>The identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. We developed a system for large-scale molecular virus screening of clinical samples based on host DNA depletion, random PCR amplification, large-scale sequencing, and bioinformatics. The technology was applied to pooled human respiratory tract samples. The first experiments detected seven human virus species without the use of any specific reagent. Among the detected viruses were one coronavirus and one parvovirus, both of which were at that time uncharacterized. The parvovirus, provisionally named human bocavirus, was in a retrospective clinical study detected in 17 additional patients and associated with lower respiratory tract infections in children. The molecular virus screening procedure provides a general culture-independent solution to the problem of detecting unknown virus species in single or pooled samples. We suggest that a systematic exploration of the viruses that infect humans, “the human virome,” can be initiated.</jats:p>

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