The Putative RNA-Dependent RNA Polymerase<i>RDR6</i>Acts Synergistically with<i>ASYMMETRIC LEAVES1</i>and<i>2</i>to Repress<i>BREVIPEDICELLUS</i>and MicroRNA165/166 in Arabidopsis Leaf Development

DOI PDF 被引用文献27件
  • Hong Li
    National Laboratory of Plant Molecular Genetics  Shanghai Institute of Plant Physiology and Ecology  Shanghai Institute for Biological Sciences  Graduate School of Chinese Academy of Sciences  Shanghai 200032  China
  • Lin Xu
    National Laboratory of Plant Molecular Genetics  Shanghai Institute of Plant Physiology and Ecology  Shanghai Institute for Biological Sciences  Graduate School of Chinese Academy of Sciences  Shanghai 200032  China
  • Hua Wang
    National Laboratory of Plant Molecular Genetics  Shanghai Institute of Plant Physiology and Ecology  Shanghai Institute for Biological Sciences  Graduate School of Chinese Academy of Sciences  Shanghai 200032  China
  • Zheng Yuan
    National Laboratory of Plant Molecular Genetics  Shanghai Institute of Plant Physiology and Ecology  Shanghai Institute for Biological Sciences  Graduate School of Chinese Academy of Sciences  Shanghai 200032  China
  • Xiaofeng Cao
    Institute of Genetics and Developmental Biology  Chinese Academy of Sciences  Beijing 100101  China
  • Zhongnan Yang
    College of Life and Environment Sciences  Shanghai Normal University  Shanghai 200234  China
  • Dabing Zhang
    College of Life Science and Biotechnology  Shanghai Jiao Tong University  Shanghai 200030  China
  • Yuquan Xu
    College of Life Science and Biotechnology  Shanghai Jiao Tong University  Shanghai 200030  China
  • Hai Huang
    National Laboratory of Plant Molecular Genetics  Shanghai Institute of Plant Physiology and Ecology  Shanghai Institute for Biological Sciences  Graduate School of Chinese Academy of Sciences  Shanghai 200032  China

抄録

<jats:title>Abstract</jats:title><jats:p>The Arabidopsis thaliana  ASYMMETRIC LEAVES1 (AS1) and AS2 genes are important for repressing class I KNOTTED1-like homeobox (KNOX) genes and specifying leaf adaxial identity in leaf development. RNA-dependent RNA polymerases (RdRPs) are critical for posttranscriptional and transcriptional gene silencing in eukaryotes; however, very little is known about their functions in plant development. Here, we show that the Arabidopsis RDR6 gene (also called SDE1 and SGS2) that encodes a putative RdRP, together with AS1 and AS2, regulates leaf development. rdr6 single mutant plants displayed only minor phenotypes, whereas rdr6 as1 and rdr6 as2 double mutants showed dramatically enhanced as1 and as2 phenotypes, with severe defects in the leaf adaxial-abaxial polarity and vascular development. In addition, the double mutant plants produced more lobed leaves than the as1 and as2 single mutants and showed leaf-like structures associated on a proportion of leaf blades. The abnormal leaf morphology of the double mutants was accompanied by an extended ectopic expression of a class I KNOX gene BREVIPEDICELLUS (BP) and high levels of microRNA165/166 that may lead to mRNA degradation of genes in the class III HD-ZIP family. Taken together, our data suggest that the Arabidopsis RDR6-associated epigenetic pathway and the AS1-AS2 pathway synergistically repress BP and MIR165/166 for proper plant development.</jats:p>

収録刊行物

  • The Plant Cell

    The Plant Cell 17 (8), 2157-2171, 2005-07-08

    Oxford University Press (OUP)

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