The cyanobacterium <i>Gloeobacter violaceus</i> PCC 7421 uses bacterial‐type phytoene desaturase in carotenoid biosynthesis

• Tohru Tsuchiya

• Shinichi Takaichi

• Norihiko Misawa

• Takashi Maoka

• Hideaki Miyashita

• Mamoru Mimuro

抄録

Carotenoid composition and its biosynthetic pathway in the cyanobacterium Gloeobacter violaceus PCC 7421 were investigated. β‐Carotene and (2 S ,2′ S )‐oscillol 2,2′‐di(α‐ l ‐fucoside), and echinenone were major and minor carotenoids, respectively. We identified two unique genes for carotenoid biosynthesis using in vivo functional complementation experiments. In Gloeobacter , a bacterial‐type phytoene desaturase (CrtI), rather than plant‐type desaturases (CrtP and CrtQ), produced lycopene. This is the first demonstration of an oxygenic photosynthetic organism utilizing bacterial‐type phytoene desaturase. We also revealed that echinenone synthesis is catalyzed by CrtW rather than CrtO. These findings indicated that Gloeobacter retains ancestral properties of carotenoid biosynthesis.

収録刊行物

• FEBS Letters

  FEBS Letters 579 (10), 2125-2129, 2005-03-11

  Wiley

キーワード

• Cell Biology
• Genetics
• Molecular Biology
• Biochemistry
• Structural Biology
• Biophysics

詳細情報

• CRID: 1362262946136595712
• NII論文ID: 80017832279
• DOI: 10.1016/j.febslet.2005.02.066
• ISSN: 18733468
• ISSN: 00145793
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2005.02.066
• Web Site: https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2005.02.066
• データソース種別:
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  • CiNii Articles