Multiple High-Throughput Analyses Monitor the Response of <i>E. coli</i> to Perturbations
-
- Nobuyoshi Ishii
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Kenji Nakahigashi
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Tomoya Baba
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Martin Robert
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Tomoyoshi Soga
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Akio Kanai
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Takashi Hirasawa
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Miki Naba
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Kenta Hirai
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Aminul Hoque
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Pei Yee Ho
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Yuji Kakazu
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Kaori Sugawara
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Saori Igarashi
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Satoshi Harada
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Takeshi Masuda
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Naoyuki Sugiyama
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Takashi Togashi
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Miki Hasegawa
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Yuki Takai
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Katsuyuki Yugi
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Kazuharu Arakawa
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Nayuta Iwata
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Yoshihiro Toya
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Yoichi Nakayama
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Takaaki Nishioka
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Kazuyuki Shimizu
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Hirotada Mori
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
-
- Masaru Tomita
- Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
書誌事項
- タイトル別名
-
- Multiple high-throughput analyses monitor the response of E. coli to perturbations.
抄録
<jats:p> Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of <jats:italic>Escherichia coli</jats:italic> cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, <jats:italic>E. coli</jats:italic> actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. <jats:italic>E. coli</jats:italic> thus seems to use complementary strategies that result in a metabolic network robust against perturbations. </jats:p>
収録刊行物
-
- Science
-
Science 316 (5824), 593-597, 2007-04-27
American Association for the Advancement of Science (AAAS)
