A Plant Peptide Encoded by <i>CLV3</i> Identified by in Situ MALDI-TOF MS Analysis

• Tatsuhiko Kondo

  Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.

• Shinichiro Sawa

  Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.

• Atsuko Kinoshita

  Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.

• Satoko Mizuno

  Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.

• Tatsuo Kakimoto

  Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.

• Hiroo Fukuda

  Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.

• Youji Sakagami

  Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.

抄録

The Arabidopsis CLAVATA3 ( CLV3 ) gene encodes a stem cell–specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ Arabidopsis tissues determined the structure of a modified 12–amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing CLV3 . These results suggest that the functional peptide of CLV3 is MCLV3.

収録刊行物

• Science

  Science 313 (5788), 845-848, 2006-08-11

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361137043973299072
• NII論文ID: 80019014311
• DOI: 10.1126/science.1128439
• ISSN: 10959203
• ISSN: 00368075
• ISSN: http://id.crossref.org/issn/00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.1128439
• データソース種別:
  • Crossref
  • CiNii Articles
  • KAKEN