Acute suppression, but not chronic genetic deficiency, of c- <i>fos</i> gene expression impairs long-term memory in aversive taste learning

• Yasunobu Yasoshima

  *Department of Behavioral Physiology, Graduate School of Human Sciences, Osaka University, 1-2 Yamada-oka, Suita 565-0871, Japan; and

• Noritaka Sako

  *Department of Behavioral Physiology, Graduate School of Human Sciences, Osaka University, 1-2 Yamada-oka, Suita 565-0871, Japan; and

• Emiko Senba

  Department of Anatomy and Neurobiology, Wakayama Medical University, 811-1 Kimiidera, Wakayama City, Wakayama 641-8509, Japan

• Takashi Yamamoto

  *Department of Behavioral Physiology, Graduate School of Human Sciences, Osaka University, 1-2 Yamada-oka, Suita 565-0871, Japan; and

抄録

Several lines of evidence have indicated that the establishment of long-term memory requires protein synthesis, including the synthesis of immediate-early gene products. Although the anatomical expression patterns of the c- fos gene, a transcription factor-encoding immediate-early gene, in conditioned taste aversion (CTA) are well documented, the functional roles of c- fos gene expression and Fos-mediated transcription remain to be clarified. Using the antisense oligodeoxynucleotide (AS-ODN) method in rats and gene-targeting knockout techniques in mice (c- fos −/− mice), we examined the roles of c- fos gene expression in the acquisition, retrieval, and retention of CTA. Preconditioning microinfusion of AS-ODN directed against c- fos mRNA (c- fos AS-ODN) into the parabrachial nucleus (PBN) impaired the acquisition, whereas infusion of control ODNs consisting of a randomized or inverted base order had no effect. Microinfusion of c- fos AS-ODN into either the amygdala or insular cortex did not impair the acquisition, whereas it attenuated the retention. Retrieval and subsequent retention of an acquired CTA were not disrupted by c- fos AS-ODN infusion into the PBN or amygdala. Microinfusion of another AS-ODN directed against zif 268 ( egr -1, krox -24, NGFI -A) mRNA into the PBN or amygdala did not affect the acquisition and retention. The genetic deficiency in c- fos −/− mice caused normal acquisition and retention. The present results suggest that the Fos-mediated gene transcription in the PBN, amygdala, or insular cortex plays critical roles in the acquisition and/or consolidation, but not the retrieval, of long-term taste memory; nevertheless, some other factors could compensate CTA mechanism when Fos-mediated transcription is not available.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 103 (18), 7106-7111, 2006-05-02

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1363107369839428352
• NII論文ID: 80019220478
• DOI: 10.1073/pnas.0600869103
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.0600869103
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