CiNii 図書 - Techniques in molecular biology

The last few years have seen the rapid development of new methodologies in molecular biology. New techniques, particularly in genetic engineering, have been introduced, and the sensitivity of older techniques improved. Volume II in this series extends material covered in volume I. As with the previous volume, the majority of chapters, relate to nucleic acid methodology, but immunological methods and protein purification techniques not covered in the first volume are also covered in Volume II. Each chapter aims to describe both the theory and relevant practical details for a given technique, identifying its potential and limitations, although the book is not intended as a 'recipe' book. It is aimed at senior undergraduates and research workers who are looking for an introductory review of a particular technique.

巻冊次: v. 2 pbk ISBN 9780709936732

内容説明

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly intro duced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have con tributed a wide range of new techniques. In Volume 1, published in 1983, we introduced the reader to a selection of the more advanced analytical and preparative techniques which we considered to be frequently used by research workers in the field of molecular biology. In choosing techniques for Volume 1 we obviously had to be selective and were unable to cover as broad a spectrum of techniques as we would have liked. However, the pro duction of Volume 2 has allowed us to develop the theme initiated in Volume 1 and also expand to include a wider range of subject areas. As with Volume 1, the majority of chapters relate to nucleic acid method ology, but we have also covered immunological methodology and protein 1. Obviously, we purification techniques that were not included in Volume see Volume 2 as simply a continuation of Volume 1. As with Volume 1, a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new termin ology, a glossary of terms has been included.

1. Micromethods in Protein Chemistry.- 2. Peptide Synthesis.- 3. Affinity Chromatography.- 4. The Enzyme Linked Immunosorbent Assay (ELISA).- 5. Monoclonal Antibodies.- 6. Plasmid Derived Cloning Vectors.- 7. The Ti-Plasmid of Agrobacterium tumefaciens as a Tool for Genetic Engineering in Plants.- 8. Cloning in Yeast.- 9. Cloning in Bacteriophage Lambda.- 10. The Dot-Blot Technique.- 11. DNA-mediated Gene Transfer into Mammalian Cells.- 12. In vitro Transcription.- 13. Chemical Synthesis of Oligodeoxyribonucleotides.- 14. Targeted Mutagenesis of Cloned DNA.- 15. DNA Footprinting and Related Techniques for Analysing Protein-DNA Interactions.- 16. Autoradiography and Fluorography.- 17. Computer Applications to Studying DNA.- List of Contributors.

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