Electron microscopy in molecular biology : a practical approach

Bibliographic Information

Electron microscopy in molecular biology : a practical approach

edited by J. Sommerville and U. Scheer

(The practical approach series)

IRL, c1987

  • : pbk

Available at  / 35 libraries

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Note

Includes index

Description and Table of Contents

Description

Vast experience has gone into providing this comprehensive guide to analysis at the ultrastructural level of nucleic acids, proteins and macromolecular complexes. Research workers will find it a unique handbook, containing procedures for applications with a wide range of biologically important macromolecules.

Table of Contents

  • Abbreviations
  • Preparation of nucleic acids for electron microscopy
  • Denaturation and hybridization of nucleic acids
  • Binding of protein to nucleic acids
  • Chromatin spreading
  • Visualizing active genes
  • Negative staining of protein molecules and filaments
  • Rotary metal shadowing for visualizing rod-shaped proteins
  • Em immunolabelling
  • Autoradiography of spread molecular complexes
  • Hybridization to chromatin and whole chromosome mounts
  • Appendix

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