Correlative microscopy in biology : instrumentation and methods

著者

• Hayat, M. A.

書誌事項

Correlative microscopy in biology : instrumentation and methods

edited by M.A. Hayat

Academic Press, c1987

注記

Includes bibliographies and index

内容説明・目次

内容説明

Correlative Microscopy in Biology: Instrumentation and Methods presents the detailed methodology of biological correlative microscopy, a technology that allows the acquisition of multiple data from single tissue block, cell, or section. The chapters in the book include detailed and complete instructions on the preparatory procedures. The book has 20 chapters that deal with various forms and systems of microscopy. Some of the forms and methods used in the book include light, scanning electron, fluorescence, scanning transmission electron, and ion microscopy, as well as combined light and electron and transmission electron microscope. Other methods and their applications are all discussed in detail in the book. This book will help students apply the methods without outside help as each methodology is presented in a step-by-step approach, including applications and techniques. Aside from students, the book will also be good reference for teachers, scientists, and researchers in the fields of biology, biochemistry, and medicine.

目次

Preface 1 Biological Specimen Preparation for Correlative Light and Electron Microscopy Introduction Methods Applications of Correlative Light and Electron Microscopy References 2 Light and Scanning Electron Microscopy in a Combined Instrument Introduction Description of the Combined Instrument Preparation and Staining Method for LM/SEM Specimens Quantitative Measurements in the LM/SEM Cathodoluminescence in the LM/SEM Biomedical Applications Developments with Another Type of Scanning Electron Microscope Conclusions References 3 Confocal Microscopy in Comparison with Electron and Conventional Light Microscopy Introduction The CSLM Instrument The Morphology of the Nucleoid of Escherichia coli Characterization of Cell Shape 3-D Imaging Techniques with CSLM Conclusion and Outlook References 4 Combined Light and Electron Microscopy of Central Nervous System Neurons and Their Afferent and Efferent Synaptic Connections Introduction Morphological Techniques: Correlative Light and Electron Microscopy of Golgi-Impregnated Neurons Immunocytochemical Techniques: Correlative Light and Electron Microscopy of Neurons Labeled Immunocytochemically for the Presence of Neuroactive Substances of Their Synthesizing Enzymes Correlative Light and Electron Microscopy of Neurons Labeled with PHA-L References 5 Intracellular Staining of Neurons in the Central Nervous System: Combined Physiological, Morphological, and Cytological Analysis of Identified Neurons Introduction Methods Tissue Processing Data Analysis Limitations of the Method Conclusions References 6 Correlative Microscopy of Catecholamines and Neuropeptides in Adrenal Medulla Introduction Vibratome Sectioning and Fluorescence Microscopy Immunocytochemistry Possible Modifications Double-Labeling Methods for Studying Coexistence Advantages and Limitations of Combined Faglu-Fluorescence and Immunocytochemistry Method References 7 Sequential Immunocytochemical and Silver Staining of Neuroendocrine Cells in the Same Section Introduction Description of Sequential Staining Technique Comments on Methodology Applications References 8 Correlative Light and Electron Microscopic Method for the Visualization of the Same in Vitro Cell Using Radioautography and Serial Sectioning Introduction Methodology Applications References 9 Correlative Light and Transmission Electron Microscopy of Mitotic Cells in the Nervous System Introduction Mitotic Cells in the Embryonic CNS Mitotic Cells in the CNS during Myelination Identification of Mitotic Cells in the Mature and Aged CNS Applications References 10 Light and Scanning Electron Microscopy of the Same Metaphase Chromosomes Introduction Methodology Applications of Correlative Light Microscopy and SEM References 11 Correlative Light and Electron Microscopy on the Same Epoxy Section Introduction Methodology Applications References 12 Correlative Microscopy of Skeletal Muscle and Endometrium Introduction Materials and Methods for Skeletal Muscle Materials and Methods for Endometrium Discussion of Methodology and Selected Results References 13 Correlative Microscopy of Isolated Glomeruli Introduction Method for Correlative Microscopy Illustrated Examples Concluding Remarks References 14 Correlative Microscopy of Cell Surface and Underlying Structures Introduction Techniques Using Light Microscopy for Cytological Features in Combination with Scanning Electron Microscopy for Surface Structures Techniques Using Backscattered and Secondary Electron Imaging on Cells and Tissues Processed for Scanning Electron Microscopy Applications and Future Prospects References 15 Correlative Light, Scanning, and Transmission Electron Microscopy of Deembedded Tissues Introduction Methodology Applications References 16 Immunoelectron Microscopic Mirror Method: A Correlative Study Introduction Methodology Correlative Light and Electron Microscopy Discussion References 17 The Detection of Amyloid Using Correlative Polarizing Light Microscopy and Electron Microscopy Introduction Amyloidosis Methods Concluding Remarks References 18 Correlative Microscopy of Human Vitreous Body Introduction Method Conclusion References 19 A New Method for Bacterial Identification by Studying the Same Section with Light Microscopy and Scanning and Transmission Electron Microscopy Introduction Light Microscopy, Scanning and Transmission Electron Microscopy as Separated Single Tool for Detecting Bacteria within Gingival Tissues Method Application References 20 Correlative Light and Scanning Electron Microscopy of Thick Sections and Isolated Cells Introduction Operating Principles Application of Correlative Scanning Electron Microscopy Stepwise Procedure Conclusion References Index

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詳細情報

• NII書誌ID(NCID): BA01418342
• ISBN:
  • 0123339227
• LCCN: 86032039
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Orlando ; Tokyo
• ページ数/冊数: xiii, 437 p.
• 大きさ: 24 cm
• 分類:
  • LCC : QH207
  • DC19 : 578
• 件名:
  • LCSH : Microscope and microscopy -- Technique