Gene cloning and analysis : a laboratory guide

Author(s)

• Boulnois, G. J. (Graham J.)
• University of Leicester

Bibliographic Information

Gene cloning and analysis : a laboratory guide

compiled by the staff of the University of Leicester gene cloning course and edited by G.J. Boulnois

Blackwell Scientific Publications, 1987

Note

Updated version of: Basic cloning techniques / edited by R.H. Pritchard and I.B. Holland. 1985

Includes bibliographies and index

Description and Table of Contents

Description

This manual is based on the experimental protocols for an intense, practical course on gene cloning and analysis held in Leicester during September 1985 and July 1986.

Table of Contents

• Translation of mRNA in a rabbit reticulocyte lysate cell-free protein synthesising system
• synthesis and cloning of cDNAs from eukaryotic mRNAs
• the isolation of cDNAs in gt 11 using antibody probes
• southern blotting
• genomic library cloning - with plasmid, and cosmid vectors
• M13 cloning and dideoxy sequencing
• oligonucleotide mutagenesis
• mapping RNA
• glossary.

by "Nielsen BookData"

Details

• NCID: BA03423751
• ISBN:
  • 0632019271
• LCCN: 87018238
• Country Code: uk
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Oxford ; Boston
• Pages/Volumes: viii, 136 p.
• Size: 28 cm
• Classification:
  • LCC : QH442.2
  • DC19 : 574.87/322
• Subject Headings:
  • LCSH : Molecular cloning -- Laboratory manuals
  • MESH : Cloning, Molecular -- methods