Biotechnology in invertebrate pathology and cell culture

著者

• Maramorosch, Karl

書誌事項

Biotechnology in invertebrate pathology and cell culture

edited by Karl Maramorosch

Academic Press, c1987

• : alk. paper

注記

Includes bibliographies and index

内容説明・目次

内容説明

Biotechnology in Invertebrate Pathology and Cell Culture provides information pertinent to genetically manipulated microbial and viral agents, which will benefit those who are interested in the development and uses of pathogens of invertebrates. This book discusses several topics, including fusion of invertebrate cells, safety of viral insecticides, and potential hazards of biocontrol agents. Organized into five parts encompassing 30 chapters, this book starts with an overview of the selection of effective strains and describes the microbial control in sericultural countries. This text then discusses the differences in crystal composition and toxicity of various subspecies, as well as the sporulation-dependent production of the crystal proteins. Other chapters explore the applications of genetically engineered organisms to biological pest control and discuss the intriguing medical applications through the utilization of invertebrate cell culture and baculoviruses. The final chapter explains the application of biotechnology to insect pathology to increase agricultural productivity. This book is a valuable resource for microbiologists, geneticists, entomologists, parasitologists, virologists, medical researchers, biocontrol researchers, and graduate students.

目次

Contributors Preface I. Genetic Manipulation of Microbial Insecticides 1. Strain Improvement of Insect Pathogens I. Bacillus thuringiensis Preparations and Sericulture II. Selection of Effective Bacillus thuringiensis Strains III. Strain Improvement of Bacillus thuringiensis IV. Use of an Attenuated Insect Virus for Control of Silkworm Nuclear Polyhedrosis V. Research Requirements for Development of Microbial Insecticides References 2. Structure and Regulation of the Crystal Protein Gene of Bacillus thuringiensis I. Introduction II. DNA Sequence Analysis III. Expression of the Crystal Protein in Escherichia coli References 3. Mechanism of Bacillus thuringiensis Insecticidal d-Endotoxin Action on Insect Cells In Vitro I. Introduction II. Materials and Methods III. Results IV. Discussion References 4. Entomocidal Activity of Crystal Proteins from Bacillus thuringiensis toward Cultured Insect Cells I. Introduction II. Cell Culture III. Toxin Bioassay IV. Modifiers of Cell Surface Activity V. Resistance VI. Conclusions References 5. Operational Bacterial Insecticides and Their Potential for Future Improvement Text References 6. Expression of d-Endotoxin Gene of Bacillus thuringiensis I. Introduction II. Experimental Results III. Conclusion References 7. Structural and Antigenic Relationships among Three Insecticidal Crystal Proteins of Bacillus thuringiensis subsp. kurstaki I. Introduction II. Materials and Methods III. Results IV. Discussion References 8. Bacillus thuringiensis Isolate with Activity against Coleoptera I. Introduction II. Materials and Methods III. Host Range IV. Biochemistry V. Molecular Genetics VI. Discussion References 9. Parasporal Body of Mosquitocidal Subspecies of Bacillus thuringiensis I. Introduction II. Parasporal Body of Bacillus thuringiensis subsp. israelenis III. Parasporal Body of Bacillus thuringiensis subsp. morrisoni (PG-14) IV. Parasporal Body of Bacillus thuringiensis subsp. darmstadiensis (73-E-l0-2) V. Closing Remarks References 10. Current Status of the Microbial Larvicide Bacillus sphaericus I. Introduction II. General Characteristics III. Development of Insecticidal Potency IV. Field Trials V. Mode of Action VI. Systematics Bacillus sphaericus VII. Biotechnology of Bacillus sphaericus VIII. Summary and Future Efforts References II. Mass Production of Microbial and Viral Insecticides 11. Production of Viral Agents in Invertebrate Cell Cultures I. Introduction II. Virus Replication in Cell Culture III. Summary and Conclusions References 12. Morphogenesis of Germinating Conidia and Protoplast-Associated Structures in Entomophthoralean Fungi I. Introduction II. Conidia in Dormant Stage III. Germ Tube Extension IV. Secondary Conidium Budding V. Capillary Tube Formation VI. Protoplast Regeneration VII. Conclusion References 13. Prospects for Development of Molecular Technology for Fungal Insect Pathogens I. Introduction II. Cloned Genes from Filamentous Fungi III. Transformation Systems for Filamentous Fungi IV. Fungal Molecular Technology V. Conclusion References III. Gene Manipulation and Cell Culture 14. Expression of Human Interferon a in Silkworms with a Baculovirus Vector I. Bombyx mori Nuclear Polyhedrosis Virus as a Vector II. Protein Synthesis in the Fat Body of the Silkworm III. Identification and Cloning of the Polyhedrin Gene of BmNPV IV. Construction of a Transfer Plasmid Vector V. Construction of a Recombinant Virus with Insertion of the Interferon Gene VI. Characteristics of Recombinant Viruses VII. Purification of the Interferon from the Silkworm VIII. Conclusion References 15. Biologically Active Influenza Virus Hemagglutinin Expressed in Insect Cells by a Baculovirus Vector I. Introduction II. Hemagglutinin: Results of Studies III. Discussion References 16. Transfection of Drosophila melanogaster Transposable Elements into the Drosophila hydei Cell Line I. Introduction II. Plasmids III. Recipient and Medium IV. Transfection and Cotransfection V. Analysis of Transfectants VI. Summary References 17. FP Mutation of Nuclear Polyhedrosis Viruses: A Novel System for the Study of Transposon-Mediated Mutagenesis I. Introduction II. Spontaneous Plaque Morphology Mutants of Nuclear Polyhedrosis Viruses III. Biological Properties of FP Mutants IV. Genetics of FP Mutants V. Transposons and Transposon-Mediated Mutagenesis of Baculoviruses VI. FP Mutation and Transposon Mutagenesis of Nuclear Polyhedrosis Viruses VII. Significance of Transposon-Mediated Mutagenesis of Baculoviruses VIII. Baculoviruses as Vectors of Genetic Elements IX. Concluding Remarks References 18. Expression of Foreign Genes in Insect Cells I. Introduction II. Baculoviruses for Commercial Production of Medically or Agriculturally Important Proteins III. Improved Baculovirus Pesticides References 19. Genotypic Variants in Wild-Type Populations of Baculoviruses I. Introduction II. Genotypic Variants of the Single-Enveloped Nuclear Polyhedrosis Viruses III. Genotypic Variants of the Multiple-Enveloped Nuclear Polyhedrosis Viruses IV. Genotypic Variants of Granulosis Viruses V. Genotypic Variants of Nonoccluded Viruses VI. Relatedness among Baculoviruses VII. Summary and Conclusions References 20. Biotechnological Application of Invertebrate Cell Culture to the Development of Microsporidian Insecticides I. Introduction II. Cell Culture III. Biotechnological Considerations IV. Safety Considerations References 21. Grasshopper and Locust Control Using Microsporidian Insecticides I. Introduction II. Materials and Methods III. Results and Discussion IV. Conclusion References 22. Establishment of Embryonic Cell Lines from the Brown Ear Tick Rhipicephalus appendiculatus and Their Immunogenicity in Rabbits I. Introduction II. Methods III. Observations IV. Summary References 23. Establishment of an Ovarian Cell Line in the Cotton Bollworm Heliothis armigera and In Vitro Replication of Its Cytoplasmic Polyhedrosis Virus I. Introduction II. Materials and Methods III. Results IV. Discussion and Conclusions References IV. Cell Fusion 24. Fusion of Insect Cells I. Introduction II. Methods for Cell Fusion and Isolation of Hybrids III. Historical Review of Insect Cell Fusion IV. Attempts to Establish Cell Fusion Methods for Insect Cells Other than Drosophila V. Epilogue References 25. Protoplast Fusion of Insect Pathogenic Fungi I. Introduction II. Materials and Methods III. Results IV. Discussion V. Summary References V. Future Perspectives 26. University-Industry Perspectives I. Introduction II. Academic Freedom vs. Needs for Confidentiality III. Product Liability IV. Division of Royalty Income V. Title to Biological Materials VI. University-Industry Agreements VII. Federal Funding VIII. Conclusion Further Readings 27. Control of Invertebrate Pests through the Chitin Pathway I. Introduction II. Substances Inhibiting the Chitin Pathway III. Exploiting the Chitinase Gene References 28. Improving the Effectiveness of Insect Pathogens for Pest Control I. Photostabilization II. Bait Formulations III. Expansion of Host Range References 29. Entomogenolls Nematodes and Their Prospects for Genetic Improvement I. Introduction II. Nematode Families of Biocontrol Importance III. Analysis IV. Prospects for Genetic Improvement V. Conclusion References 30. Genetically Engineered Microbial and Viral Insecticides: Safety Considerations Text References Index

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詳細情報

• NII書誌ID(NCID): BA03895309
• ISBN:
  • 0124702554
• LCCN: 87001206
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: San Diego ; Tokyo
• ページ数/冊数: xx, 511 p.
• 大きさ: 24 cm
• 分類:
  • LCC : TP248.I57
  • DC19 : 668/.651
• 件名:
  • LCSH : Microbial insecticides
  • LCSH : Viral insecticides
  • LCSH : Microbial insecticides industry
  • LCSH : Viral insecticides industry
  • LCSH : Microbial genetics
  • MESH : Cells, Cultured -- drug effects
  • MESH : Genetic Intervention
  • MESH : Genetics, Microbial
  • MESH : Insecticides -- pharmacodynamics
  • MESH : Insects -- genetics