Progress in plant protoplast research : proceedings of the 7th International Protoplast Symposium, Wageningen, the Netherlands, December 6-11, 1987

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Progress in plant protoplast research : proceedings of the 7th International Protoplast Symposium, Wageningen, the Netherlands, December 6-11, 1987

edited by K.J. Puite ... [et al.]

（Current plant science and biotechnology in agriculture, 7）

Kluwer Academic Publishers, 1988

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Includes bibliographies andindex

内容説明・目次

内容説明

In the series of International Protoplast Symposia the Symposium of 1987 was held in Wageningen (The Netherlands). Earlier Symposia took place in Jena (DDR) 1963, Brno (CSSR) 1967, Salamanca (Spain) 1971, Nottingham (UK) 1975, Szeged (Hungary) 1979 and Basel (Switzerland) 1983. This 7th International Protoplast Symposium was organized by K.J. Puite (Secretary), J.J.M. Dons (Treasurer), H.J.Huizing and E.J.L. Hotke-Staal (Local Organizers), the first three persons being scientists, respectively, from the Research Institute Ital, the Institute for horticultural plant breeding IVT and the Foundation for agricultural plant breeding SVP at Wageningen. Scientific Advisers of the Symposium were A. J. Kool, M. Koornneef and F.A. Krens. The International Agricultural Centre lAC served as the Symposium location. The Organizing Committee decided that the scientific programme of the Symposium should be mainly focussed on protoplast technology of relevance to plant breeding. Therefore research on microbial protoplasts and on secondary metabolites was not included. About 250 scientists from 27 different countries were welcomed at the meeting. Speakers at Symposium Sessions and authors of Poster contributions were asked to hand over their manuscripts for the Symposium Proceedings already at the meeting, permit ting early publication of the Proceedings. These manuscripts give the state of the art of the protoplast research and illustrate the progress since the last Protoplast Symposium.

目次

  • Progress in Plant Protoplast Research.- Obstacles and Perspectives in Protoplast Research.- Aspects of Protoplast Culture and Plant Regeneration.- Recent Developments in the Regeneration of Agronomically Important Crops from Protoplasts.- Selection, Experimental Manipulation and Culture of Single Protoplasts.- Uniparental, Fusant, and Chimeric Plants Regenerated from Protoplasts after Streak Plating in Agarose Gels.- Electroporation Mediated Enhancement of Plant Protoplast Division and Plant Regeneration.- Selection of Potato Cell Suspension Protoplast Subpopulation by Rhodamine 123.- Protoplast Culture of Dioscorea opposita Thunb. (D. Batatas Decne).- Isolation and Culture of Buckwheat (Faqopyrum esculentum Moench.) Callus Protoplasts.- Partial Purification of a Cellulase Used for Protoplast Isolation from Barley (Hordeum vulgare).- Plant Regeneration from Cell Suspension Protoplasts of Festuca arundinacea Schreb., Lolium perenne L. and L. multiflorum Lam..- Isolation, Culture and Regeneration of Lolium perenne and Lolium multiflorum Protoplasts.- Mature Plant Regeneration from cultivated Protoplasts of Rice (Oryza sativa L.).- The Plants Regenerated from Protoplasts of Suspension Cells of Rice (Oryza sativa L).- Regeneration of Plantlets from Protoplasts of Poa pratensis L. (Kentucky Bluegrass).- Somatic Embryogenesis and Plant Regeneration from Protoplast of Maize (Zea mays L.).- Protoplasts from Actinidia deliciosa (Kiwi): Obtention and Culture.- Colony Formation from Cell Suspension Culture Protoplasts of Kiwifruit.- Isolation and Culture of Grapevine Protoplasts (Vitis Vinifera L.).- Studies Towards a Protoplast Isolation and Culture Procedure in Sugarbeet (Beta vulgaris L.) Applicable to a Variety of Genotypes.- Improvement of Protoplast Culture in Helianthus annuus (L).- Factors Influencing Protoplasts Plating Efficiency and Direct Somatic Embryogenesis Induction in Sunflower (Helianthus annuus L.).- Comparison of Sunflower Protoplast Development in Liquid or Solidified Media.- Plant Regeneration from Protoplasts of Rudbeckia and Dimorphotheca Species (Compositae).- Optimization of Lettuce Calli Regeneration from Protoplasts.- Optimal Density and Osmolality of Protoplasts for Improved Cultural Performance.- Development of Protoplast Culture Procedures in Brassica Crops Suitable for in vitro Selection Herbicide Resistance Mutants and Cybrids.- Somatic Embryogenesis in Stationary Phase Suspension Cultures Derived from Hypocotyl Protoplasts of Brassica napus L..- Plant Regeneration from Cucumber Protoplasts.- Callus Formation from Mesophyll protoplasts of Forage Legume Astragalus huangheensis Fu et Lin.- Preliminary Studies of Lupinus Protoplasts.- Regeneration and Differentiation of Protoplasts in Grain Legumes.- Plant Regeneration from Strawberry (Fragaria ananassa) Mesophyll Protoplasts.- Isolation and Culture of Apple Mesophyll Protoplasts.- Phizogenesis as an Intermediate Step Towards Plant Regeneration of Protoolasts of Sour Cherry (Prunus cerasus L.) Rosaceae.- Isolation and Culture of Protoplasts from Totipotent Cell Cultures of Populus ciliata.- A Protoplast System for Populus which will Support Genetic Engineering Research of Tree Species.- Factors Influencing Digitalis obscura Mesophyll Protoplast Development.- Cotyledon Protoplasts from Solanum melongena L. Regeneration to Fertile.- Isolation, Culture and Regeneration of Leaf Protoplasts in the Genus Capsicum.- Isolation and Properties of Protoplasts of Theobroma cacao L..- Plantlet Regeneration from Protoplasts of Levisticum officinale Koch. and its Cytological Investigation.- Differences in Developmental Morphology of Protoplasts from Two Cell lines of which Spruce (Picea glauca).- Protoplast Development from Porphyra linearis - An Edible Marine Red Alga.- Structure and Physiology of the Protoplast Plasma Membrane.- Why do Mesophyll Protoplasts Dedifferentiate?.- Release of Phytotoxic Factors from Plant Cell Walls During Protoplast Isolation.- Role of Peroxidase in Protoplast Development: The Activity and Molecular Forms of Peroxidase in Mitogenic and Non-Mitogenic Protoplasts.- Measurement of Cytoplasmic CA2+ and H+ in Barley Aleurone Protoplasts.- The Effect of Secretion on Lipids and Their Distribution in Barley Aleurone Protoplasts.- Incidence of Growth Regulators on RNA/DNA Levels in Protoplasts as Analysed by Acridine Orange Staining and Flow Cytometry.- IAA, ABA Content and Metabolism in Maize Root Protoplasts.- Some Metabolic Peculiarities of Isolated Protoplasts.- Gene Transfer by Individual Manipulation of Plant Cells.- Potentials of Protoplast Fusion in Plant Breeding Programmes.- Use of Protoplasts in Plant Breeding: Cytoplasmic Aspects.- Protoplast Fusion between Eruca sativa and Brassica napus - Analysis of Regenerated Plants.- Fertility and Chromosome Stability in Brassica napus Resynthesised by Protoplast Fusion.- Involvement of Protoplast Fusion in Cauliflower Breeding.- Protoplast Fusion as a Method of Increasing Cytoplasmic Heterogeneity in Brassica napus.- Selection of a Universal Hybridizer in Sinapis turgida Del. and Regeneration on Plantlets from Somatic Hybrids with Brassica species.- Towards the Introduction of Cytoplasmic Male Sterility (CMS) into Brassica napus Through Protoplast Fusion.- Production of Somatic Hybrid Plants, 'Brassicnmoricandia', Through Protoplast Fusion between Moricandia arvensis and Brassica oleracea.- The Use of Haploid Protoplast Fusion to Combine Cytoplasmic Atrazine Resistance and Cytoplasmic Male Sterility in Brassica napus.- Somatic Hybridization of Diploid Potato Lines, A Tool in Potato Breeding.- Somatic Hybridization between Anther-Derived Dihaploid Lines of Solanum tuberosum L. (Potato).- Fusion of Dihaploid Solanum tuberosum Protoplasts and Trials of Hybrid Selection with Phytotoxins.- Production and Field Assessment of Somatic Hybrids between Dihaploid Solanum tuberosum and Solanum brevidens.- Fusion of Solanum tuberosum L. cv. Explorer (Pototo) and Solanum melongena L. cv. Imperial Black Beauty (Eggplant) Protoplasts and Subseguent Callus Regeneration.- Somatic Fusions of Solanum Species.- Flow Cytometric Sorting of Potato Heterokaryons with Inhibition of Cell Wall Synthesis Before Sorting.- An Improved Peg Fusion Procedure for Flow Sorting of Plant Protoplasts.- Regeneration and Evaluation of Somatic Hybrid Plants between Solanum tuberosum and Lycopersicon pimpinellifolium.- Studies of Gene Mapping in the Potato Using Somatic Hybrids of Solanum tuberosum L. + Nicotiana plumbaginifolia.- Interspecific Expression of AEC-Resistance in Somatic Hybrids of AEC-Resistant Solanum tuberosum and Nitrate-Reductase-Deficient Nicotiana plumbaginifolia.- Interspecific Protoplast Fusion for the Transfer of Atrazine Resistance from Solanum nigrum to Tomato (Lycopersicon esculentum. L.).- Somatic Hybridization between Tomato and Other Lycopersicon or Solanum Species.- Transfer of Regeneration Capacity from Lycopersicon peruvianum to L. esculentum by Protoplast Fusion.- Somatic Hybridization in Lycopersicon
  • Using Gamma-Irradiated Nicotiana tabacum SR-1 or Lycopersicon peruvianum.- Regeneration of Somatic Hybrid Plants between Tobacco (Nicotiana tabacum L.) and Black Nightshade (Solanum nigrum L.).- The Use of Streptomycin Resistance and Chlorophyll Deficiency for Selection of Somatic Hybrids Between Nicotiana tabacum and N. rustica.- Intra- and interspecific Gametosomatic Hybridisation Within the Genus Petunia.- The Transfer of Cytoplasmic Male Sterility (CMS) by Intraspecific Somatic Hybridisation in Petunia hybrida (Hort) VILM.- Applimation of Protonlast Fusion to an Ornamental Breeding Programme.- Formation of Carrot Cybrids Based on Metabolic Complementation by Fusion between X-Irradiated Cells and Iodoacetamide-Treated Cells.- Optimization of Conditions for Somatic Cybridization of Daucus carota L..- Isolation and Fusion of Apium graveolens (Celery) and Levisticum officinale (Lovage) Mesophyll Protoplasts.- Electrofusion of Plant and Fungal Protoplasts.- Regeneration and Analysis of Callus from Flow-Sorted Heterokaryons of Soybean (Glycine max) and G. canescens.- Interspecific Somatic Hybridization Studies in the Genus Lotus.- Interspecific Hybrid Lines of M. sativa and M. arborea by Protoplast Electrofusion.- Electrofusion and Electroporation of Sugar Beet (Beta vulgaris L.) Protoplasts.- The Production and Electrofusion of Beta cytoplasts.- Inactivation of Sugarbeet Protoplasts Using Acridin Orange, an Agent for Late Selection of Fusion Products.- Mitochondrial DNA Amplification in Somatic Hybrid Cells of Grasses.- Plant Regeneration from Electrofused Protoplasts of Chrysanthemum morifolium and Salpiglossis sinuata.- Electrofusion of Chemically-Aggregated Protoplasts Derived from Mesophyll Tissue and Habituated Cells of Catharanthus roseus.- Studies on the Tranfer of the Mitochondrial Genome Through Protoplast Fusion in Rice.- Increase of Genetic Variability by Asymmetric Cell Hybridization and Isolated Chromosome Transfer.- Cell-Mediated Gene Transfer.- Protoplast Fusion of Kanamycin Resistant, CNX- Nicotiana plumbaginifolia with Streptomycin Resistant N. tabacum (SR1) and the Effect of Irradiation of the N. tabacum Parent..- Selection for Resistance to Phoma Lingam Using Pycnospore Infections of Plants In-Vivo and Sirodesmin PL as Selective Agent In-Vitro.- Induction of Micronuclei by Amiprophos-Methyl and Their Application for Transfer of Single, Specific Chromosomes in Plant Cells.- Isolation of Microprotoplasts.- Direct Cell to Cell Transfer of Mitochondria by Microinjection.- Classification and Sorting of Plant Chromosomes Using Flow Cytometry.- Application of Flow Cytometry for the Isolation of Metaphase Chromosomes from Protoplasts of Nicotiana plumbaginifolia.- Electroporation for DNA Delivery to Plant Protoplasts.- Electromanipulation of Protoplasts.- Plant Protoplast Electropermeabilization: Role of Electrical and Biological Parameters.- The Fusogenic State of Plant Protoplasts as Related to DYE/DNA Uptake.- Genetic Manipulation of Plant Cells and Organelles with a Laser Microbeam.- Electric Field Mediated DNA Uptake and Gene Expression in Mesophyll, Suspension Culture, and Tuber Protoplasts of Wild and Cultivated Potato.- of Selectable Markers into Different Diploid Potato (Solanum tuberosum L.) Genotypes by Direct Gene Transfer.- Direct Gene Transfer in Petunia hybrida Protoplasts: Evidence for Co-Transformation.- Electroporation of Tobacco Protoplasts: A. Model System to Study Plant Gene Expression.- Infection of Tobacco Protoplasts with RNA of a Pepper Strain of Tobacco Mosaic Virus by Electroporation.- Comparison of Direct Gene Transfer Into Nicotiana Protoplasts by Electroporation and Mg2+/PEG Methods.- Quantification of Transient Expression Levels of Genes Transferred to Plant Protoplasts by Electroporation.- Gene Transfer by Electroporation in Alnus incana Protoplast.- Transformation of Rice Protoplasts by Direct DNA Uptake.- Isolation, Culture and Morphogenesis from Wheat Protoplasts, and Study of Expression of DNA Constructs by Direct Gene Transfer.- UV Inducible Transient Expression of Chimeric CHS-NPTII in Parsley Protoplasts.- Modification of Plants Using Intracellular Associations of L-Form Bacteria.- Transformation of Brassica nigra through Protoplast Cocultivation with Agrobacterium tumefaciens.- Transformation of Brassica Species via Direct Gene Transfer.- Genetic Transformation of Diploid Solanum tuberosum by Direct DNA Transfer and Mediated by Agrobacterium tumefaciens.- Optimization of Direct Gene Transfer in Tomato.- Transformation Studies in Tobacco, Moth Bean and Brassica Species.- Transposition and Regulation of the EN Elements of Maize Assayed in Transformed Tobacco.- Initiation of Cell Suspensions, Protoplast Culture, and Direct Gene Transfer to Protoplasts of Secale cereale L. (Rye).- Protoplast Culture and DNA Uptake in Echinocloa.- Transient Expression of Foreign Genes in Wheat Protoplasts.- Protoplast Culture and Transformation Studies of Triticale (x Triticosecale Wittmack).- Analysis of the Zea Mays Sucrose Synthase Promotor by Transient Gene Expression in Maize Protoplasts.- Protoplast Culture of 2n S. tuberosum as a Source of Mutant Phenotypes.- Cytoplasmic or Nuclear Control of Male Sterility in Protoolast-Derived Plants of Nicotiana sylvestris.- Selection and Characterization of Nitrate Reductase-Deficient Mutants of Nicotiana plumbaqinifolia.- Isolation of Temperature-Sensitive Auxin-Requiring Variants of Nicotiana plumbaginifolia.- The Use of Phospinothricin Resistance as a Selectable Marker in Tobacco Protoplast Transformation.- Selection for Salt/Drought Tolerance Using Isolated Protoplasts and Protoplast-Derived Calli of Colt Cherry (Prunus avium X Pseudocerasus).- Field Tests of Progeny from Protoplast Regeneration Oilseed Rape.- Studies of Lettuce Plants Regenerated from Protoplasts.- Somaclonal Variations for Indole Alkaloid Accumulation in Protoclones Derived from an Habituated Strain of Catharanthus roseus.- Somaclonal Variations of Dihydrofuroquinoline Alkaloids in Cell Lines Issued from Strains and Leaf Mesophyll of Choisya Ternata by Protoplast Cloning.- Some Aspects of Salinity Tolerance of Calli Derived from Leaf Tomato Protoplasts.- Proto-Variation in Protoplast Derived Brassica napus Plants.- Index of Organism.- Index of Authors.

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