Molecular biology of RNA : new perspectives

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書誌事項

Molecular biology of RNA : new perspectives

edited by Masayori Inouye and Bernard S. Dudock

Academic Press, c1987

  • alk. paper

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注記

Includes index

内容説明・目次

内容説明

Molecular Biology of RNA: New Perspectives provides an overview of the developments in RNA research as well as the approaches, strategies, and methodologies used. Most of the contributing authors in the present volume participated in the Fifth Stony Brook Symposium entitled "New Perspectives on the Molecular Biology of RNA" in May 1986. The text is organized into six parts. Part I contains papers dealing with RNA as an enzyme. Part II presents studies on RNA splicing. Part III examines RNA viruses while Part IV focuses on the role of RNA in DNA replication. Part V is devoted to the structure, function, and isolation of RNA. Finally, Part VI takes up the role of RNA in regulation and repression. This volume will help provide new direction and insight for those already working on the subject and will serve as a useful guide to those about to start research in the molecular biology of RNA.

目次

  • Preface I RNA as an Enzyme 1 Cleavage of RNA by RNAse P from Escherichia coli I. Introduction II. Aspects of the RNase P Reaction III. Studies of Enzyme-Substrate Interactions IV. Structure-Function Relationships in Ml RNA V. Studies of the Protein Subunit of RNase P VI. Hybrid Enzymes VII. Conclusion References 2 Bacillus Subtilis RNase I. The RNase P Components II. Mechanism of RNase P Cleavage III. Toward the Higher-Order Structure of RNase P RNA IV. Structure-Function Relationships in the RNase P RNA V. Why Is the Catalytic Element of RNase P Composed of RNA? References 3 Multiple Enzymatic Activities of an Intervening Sequence RNA from Tetrahymena I. Introduction II. Self-Splicing RNA III. The IVS RNA Enzyme References 4 Processing and Genetic Characterization of Self-Splicing RNAs of Bacteriophage I. Introduction II. Group I Splicing Mechanism for T4 td RN III. Nondirected Mutagenesis and Delineation of Two Functional Domains for Splicing in the td Intron IV. Multiple Self-Splicing Introns in T4 V. Conclusions References II RNA Splicing 5 The Mammalian Pre-Messenger RNA Splicing Apparatus: A Ribosome in Pieces? I. Introduction II. The Discovery of snRNPs III. The snRNPs-and-Splicing Hypothesis IV. snRNP Components and Structure V. Is Eukaryotic RNase P an Sm snRNP? VI. U1 snRNPs Bind 5' Splice Sites VII. U2, U5, and U4/U6 snRNPs Also Participate in Splicing VIII. The Spliceosome-Ribosome Analogy References 6 Exon Sequences and Splice Site Proximity Play a Role in Splice Site Selection I. Introduction II. Exon Sequences and Splice Site Proximity Play a Role in Splice Site Selection III. The Pattern of Splice Site Selection Is Altered in Different Extract Preparations and in Diluted Extracts IV. Splice Site Selection Can Be Altered by Competition in Trans V. Discussion References 7 Factors That Influence Alternative Splice Site Selection in Vitro I. Introduction II. Materials and Methods III. Results IV. Discussion References 8 Messenger RNA Splicing in Yeast I. An Overview of Nuclear mRNA Splicing II. Preliminary in Vitro and in Vivo Characterization of Yeast mRNA Splicing III. Characterization of Mutations in the Splicing Process IV. The RNA Gene Products and the Spliceosome V. Speculations References 9 Architecture of Fungal Introns: Implications for Spliceosome Assembly I. Introduction II. Branch Site-3' Splice Junction Relationship III. Branch Site-5' Splice Junction Relationship IV. Perspectives References 10 RNA Joining and Trypanosome Gene Expression I. Introduction II. Materials and Methods III. Results IV. Discussion V. Summary References III RNA Viruses 11 The Polio virus Genome: A Unique RNA in Structure, Gene Organization, and Replication I. Introduction II. Translation and Processing of the Polyprotein III. RNA Replication IV. Conclusions References 12 Permanent Expression of Influenza Virus Genes Coding for Transcriptase Complexes: Complementation of Viral Mutants I. Establishment of a Functional Expression System II. Addition of Nuclear Protein to the Transcription Complex III. Conclusions and Outlook References 13 Molecular Mechanisms of Pathogenesis Text References IV RNA in DNA Replication 14 Changes in RNA Secondary Structure May Mediate the Regulation of IncFII Plasmid Gene Expression and DNA Replication I. Introduction II. RNA Secondary Structure Predictions III. Discussion References 15 Regulation of Co IE 1 DNA Replication by Antisense RNA I. Introduction II. RNA Primer Formation III. Regulation of Primer Formation IV. Binding of RNA I to RNA II V. Importance of the Rate of Binding of RNA I to RNA II VI. Secondary Structure of RNA II and Its Alteration by Binding of RNA I VII. Conclusions References 16 A Transfer RNA Implicated in DNA Replication I. Introduction II. Expression of the DNA Y Gene III. Implication in DNA Replication IV. Summary and Models for Replication Role References V RNA: Structure, Function, and Isolation 17 Stable Branched RNA Covalently Linked to the 5' End of a Single-Stranded DNA of Myxobacteria I. Introduction II. DNA Structure of Stigmatella aurantiaca msDNA III. RNA Sequence of RNA-Linked msDNA IV. Determination of the Linkage between RNA and msDNA V. Gene Arrangement of the Coding Regions for msDNA (msd) and msdRNA (msr) on the Chromosome VI. Biosynthesis of Branched RNA-Linked msDNA VII. How Is Branched RNA-Linked msDNA Synthesized? References 18 Recognition of RNA by Proteins I. A Simple RNA-Protein Interaction II. A Complex RNA-Protein Interaction References 19 A New Role for Transfer RNA: A Chloroplast Transfer RNA Is a Cofactor in the Conversion of Glutamate to Delta-Aminolevulinic Acid I. Introduction II. RNA DALA Is a Unique Glutamine-Accepting tRNA III. RNA as a Cofactor: Possible Reaction Mechanism IV. Outlook References 20 Natural Suppressor Transfer RNA in Eukaryotes: Its Implication in the Evolution of the Genetic Code and Expression of Specific Genes I. Introduction II. Isolation from Tetrahymena of the tRNA Gene and tRNA Corresponding to the Termination Codon UAA III. Deviation of the Genetic Code of Tetrahymena from the Universal Genetic Code IV. Evolution of Glutamine tRNAs Recognizing UAA and UAG Termination Codons in Tetrahymena V. Isolation of a Natural UAG Suppressor Glutamine tRNA from Mouse Cells VI. A Large Increase of tRNAg[
  • UG in Mouse Cells Infected with Mo-MuLV VII. Concluding Remarks References 21 The Purification of Small RNAs by High-Performance Liquid Chromatography I. Introduction II. Materials and Methods III. Results IV. Discussion References 22 Comparative Studies on the Secondary Structure of the RNAs of Related RNA Coliphages I. Introduction II. Results III. Discussion References VI RNA in Regulation and Repression 23 Autogenous Regulation of Transcription of the crp Operon by a Divergent RNA Transcript I. Introduction II. Activation of a Divergent Promoter by Cyclic AMP-CRP is Required for crp Autoregulation III. Autoregulation of crp Is Mediated by Divergent RNA IV. A Model for crp Autoregulation V. Conclusion References 24 The Role of Translational Regulation in Growth Rate-Dependent and Stringent Control of the Synthesis of Ribosomal Proteins in Escherichia Coli I. Secondary Structure of the LI Target Site on L11 mRNA II. Growth Rate-Dependent Control of Ribosomal Protein Synthesis III. Stringent Control of Ribosomal Protein Synthesis IV. Concluding Remarks References 25 Sequence and Structural Elements Associated with the Degradation of Apolipoprotein II Messenger RNA I. Introduction II. Effect of 5' Noncoding Sequences on apo II mRNA Stability III. Use of Enzymatic Probes and Reverse Transcriptase to Analyze apo II mRNA Secondary Structure IV. Analysis of apo II mRNA in Polysomes V. Summary and Perspective References 26 A New Immune System against Viral Infection Using Antisense RNA: micRNA-Immune System I. Introduction II. micRNA Mutagenesis III. micRNA-Immune System IV. Conclusion References 27 Regulation of IS10 Transposase Expression by RNA/RNA Pairing I. Introduction II. Biological Role of Multicopy Inhibition III. Molecular Mechanism of Multicopy Inhibition IV. Mechanism of Pairing between RNA-IN and RNA-OUT In Vitro V. In Vivo Phenotypes of Mutations in RNA-OUT References 28 Characterization and Functional Analysis of the Factors Required for Transcription of the Adenovirus Major Late Promoter I. Introduction II. Fractionation and Functional Analysis of the Factors Required for Transcription of the Adenovirus Major Late Promoter III. Concluding Remarks References Index

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