Plant genetic transformation and gene expression : a laboratory manual
著者
書誌事項
Plant genetic transformation and gene expression : a laboratory manual
Blackwell Scientific Publications, 1988
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注記
Includes bibliographies and indexes
内容説明・目次
内容説明
Based on the successful course in Plant Genetic Transformations and Gene Expression run at Leicester University, this laboratory manual describes methods for the genetic transformation of plants and provides instruction on the preparation of plant material. For easy use at the laboratory bench, the methods are clearly laid out and the manual has a spiral binding and laminated covers. This book will be invaluable to academic and industrial researchers wishing to study plant gene expression or genetic engineering using recombinant DNA technology.
目次
- Contents: Vectors for the transformation of plant cells using Agrobacterium
- Transformation of dicotyledenous plant cells using the Ti plasmid of Agrobacterium tumefaciens and the Ri plasmid of A. rhizogenes
- Transformation of plant cells by DNA-mediated gene transfer
- The isolation of plant nucleic acids
- DNA restriction and analysis by Southern hybridization
- Analysis of gene organization and expression in plants.
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