Microbial toxins : tools in enzymology

書誌事項

Microbial toxins : tools in enzymology

edited by Sidney Harshman

(Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 165)

Academic Press, c1988

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注記

Includes bibliographies and indexes

内容説明・目次

内容説明

Microbial toxins are useful as agents in studies on membrane structure, biochemical pathways, and cellular regulatory mechanisms. Since relatively few of these toxins are commercially available, investigators must prepare their own reagents. This book provides in a single source the methods for preparing specific microbial toxins and their assay. It demonstrates their usefulness as reagents in biochemical and physiological studies, particularly at the cellular and molecular levels.

目次

Preparation of Toxins: Gram-Positive Cocci: S. Harshman, N. Sugg, and P.Cassidy, Preparation and Purification of Staphylococcal Alpha Toxin. M. Plommet, Preparation and Purification of ~gg-Hemolysin of Staphylococci. T.H. Birkbeck and J.H. Freer, Purification and Assay of Staphylococcal ~gd-Lysin. M. Noda and I. Kato, Purification and Crystallization of Staphylococcal Leukocidin. J.C.S. de Azavedo, C.J. Bailey, and J.P. Arbuthnott, Purification of Epidermolytic Toxin of Staphylococcus aureus. D.A. Blomster-Hautamaa and P.M. Schlievert, Preparation of Toxic Shock Syndrome Toxin-1. J.J. Iandolo and R.K. Tweten, Purification of Staphylococcal Enterotoxins. J.E. Alouf and C. Geoffroy, Production, Purification, and Assay of Streptolysin O. J.E. Alouf and C. Loridan, Production, Purification, and Assay of Streptolysin S. C. Geoffroy and J.E. Alouf, Production, Purification, and Assay of Streptococcal Erythrogenic Toxin. Gram-Positive Bacilli: S.F. Carroll, J.T. Barbieri, and R.J. Collier, Diphtheria Toxin: Purification and Properties. L.L. Simpson, J.J. Schmidt, and J.L. Middlebrook, Isolation and Characterization of the Botulinum Neurotoxins. J.P. Robinson, Purification of Tetanus Toxin and Its Major Peptides. C. Jolivet-Reynaud, H. Moreau, and J.E. Alouf, Purification of Alpha Toxin from Clostridium perfringens: Phospholipase C. J.L. McDonel and B.A. McClane, Production, Purification, and Assay of Clostridium perfringens Enterotoxin. S.H. Leppla, Production and Purification of Anthrax Toxin. Gram-Negative Cocci: A.G. Plaut, Production and Isolation of Neissereal IgA1 Proteases. V.Y. Perera, Purification of Pertussis Toxin. Gram-Negative Bacilli: R.N. Greenberg and A.M.K. Saeed, Purification of Escherichia coli Heat-Stable Enterotoxin. G.A. Bohach, S.J. Cavalieri, and I.S. Snyder, Purification of Escherichia coli ~ga-Hemolysin. K.J. Kozak and C.B. Saelinger, Preparation of Pseudomonas Exotoxin A. G.T. Keusch, A. Donohue-Rolfe, M. Jacewitz, and A.V. Kane, Shigella Toxin: Production and Purification. T.C. Montie, Preparation of Yersinia pestis Plague Murine Toxin. Vibrios: J.J. Mekalanos, Production and Purification of Cholera Toxin. A.S. Kreger, M.H. Kothary, and L.D. Gray, Cytolytic Toxins of Vibrio vulnificus and Vibrio damsela. Y. Takeda, Thermostable Direct Hemolysin of Vibrio parahaemolyticus. J.T. Buckley and S.P. Howard, Aerolysin from Aeromonas hydrophila. ~akA. Ljungh and T. Wadstrvm, Preparation of Hemolysin from Aeromonas. Immunotoxins: T.N. Oeltmann and R.G. Wiley, Preparation of Diphtheria Toxin Fragment A Coupled to Hormone. Assay of Toxins: A.W. Bernheimer, Assay of Hemolytic Toxins. S.F. Carroll and R.J. Collier, Diphtheria Toxin: Quantification and Assay. C.B. Saelinger, Use of Exotoxin A to Inhibit Protein Synthesis. A. Dohohue-Rolfe, M. Jacewicz, and G.T. Keusch, Shigella Toxin as Inhibitor of Protein Synthesis. D.M. Gill and M. Woolkalis, [32P]ADP-Ribosylation of Proteins Catalyzed by Cholera Toxin and Related Heat-Labile Enterotoxins. M. Woolkalis, D.M. Gill, and J. Coburn, Assay and Purification of Cytosolic Factor Required for Cholera Toxin Activity. R.G. Wiley, A. Donohue-Rolfe, G.T. Keusch, and T.N. Oeltmann, Selective Destruction of Vagal Sensory Neurons Using Shigella Cytotoxin. J.J. Lipman, Measurement of Neurotoxic Actions on Mammalian Nerve Impulse Conduction. J.J. Lipman, Electroencephalogram as A Tool for Assaying Neurotoxicity. M. Thelestam, Assay of Pore-Forming Toxins in Cultured Cells Using Radioisotopes. R. F

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  • Methods in enzymology

    editors in chief, Sidney P. Colowick, Nathan O. Kaplan

    Academic Press c1955-

    v. 1 , v. 2 , v. 3 , v. 4 , v. 5 , v. 6 , v. 7 , v. 33 , v. 75 , v. 95 , v. 120 , v. 140 , v. 175 , v. 199 , v. 229 , v. 265 , v. 285 , v. 320 , v. 355

    所蔵館178館

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