Methods in plant molecular biology
Author(s)
Bibliographic Information
Methods in plant molecular biology
Academic Press, c1989
- : pbk.
Available at 19 libraries
Note
Includes index
Description and Table of Contents
Description
Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom.
Table of Contents
Laboratory Schedule.Restriction Mapping of Plasmid DNA.Cloning of Restriction Fragments into pUC Vectors.Preparation of Intact Chloroplasts from Pea.Lowry Assay for Protein Determination.Protein Synthesis by Isolated Pea Chloroplasts.Separation of Thylakoid and Stomal Proteins by SDS-Gel Electrophoresis.Isolation of Chloroplast DNA.RNA Isolation from Light- and Dark-Grown Seedlings.Preparation of a Wheat Germ Extract for in Vitro Translation of mRNA.Screening of Recombinant Phage Libraries with Cloned cDNA Probes.Isolation of Phage DNA from Liquid Cultures.Dideoxy DNA Sequencing.Transformation of Leaf Discs with Agrobacterium.Glossary.Gel Electrophoresis Equipment
by "Nielsen BookData"