Methods in plant molecular biology
著者
書誌事項
Methods in plant molecular biology
Academic Press, c1989
- : pbk.
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注記
Includes index
内容説明・目次
内容説明
Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom.
目次
Laboratory Schedule.Restriction Mapping of Plasmid DNA.Cloning of Restriction Fragments into pUC Vectors.Preparation of Intact Chloroplasts from Pea.Lowry Assay for Protein Determination.Protein Synthesis by Isolated Pea Chloroplasts.Separation of Thylakoid and Stomal Proteins by SDS-Gel Electrophoresis.Isolation of Chloroplast DNA.RNA Isolation from Light- and Dark-Grown Seedlings.Preparation of a Wheat Germ Extract for in Vitro Translation of mRNA.Screening of Recombinant Phage Libraries with Cloned cDNA Probes.Isolation of Phage DNA from Liquid Cultures.Dideoxy DNA Sequencing.Transformation of Leaf Discs with Agrobacterium.Glossary.Gel Electrophoresis Equipment
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