Quantitative bioassay
著者
書誌事項
Quantitative bioassay
（Analytical chemistry by open learning）
Published on behalf of ACOL, Thames Polytechnic, London, by Wiley, c1987
- : pbk.
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注記
Bibliography: p. xix-xxi
内容説明・目次
内容説明
Quantitative Bioassay covers the practical aspects of the different assays available, introduces immunology and assesses the merits of alternative methods of analysis. There is a very useful section on experimental design and a discussion on the use of assays for quantitative analysis. The book includes a description of the properties and production of polyclonal and monoclonal antibodies which should prove particularly useful to chemists and biochemists with no training in immunology who find themselves using these methods, many of which are available in kit form. It will also serve as a useful basis for further reading by anyone undertaking the establishment of new methods themselves.
目次
- GENERAL ASPECTS OF BIOASSAYS: The Use of Animals and Animal Tissues
- Biological Activity and Standard Materials
- Types of Effects
- Experimental Design
- Evaluation of Assays
- MICROBIOLOGICAL ASSAYS: Comparisons of Microbiological Assays with Bioassays using Animals
- Basic Approaches
- Plate and Tube Assays
- INTRODUCTORY IMMUNOLOGY: Antibodies and Antigens
- Characteristics of Antigens and Antibodies
- THE NATURE AND PRODUCTION OF BINDING AGENTS OF BIOLOGICAL ORIGIN: Haptens
- Limitations of Antibodies as Analytical Reagents
- Polyclonal Antibody Production
- Monoclonal Antibodies
- APPLICATIONS OF BIOLOGICAL BINDING AGENTS IN QUANTITATIVE ANALYSIS: Methods Involving Changes Secondary to Binding
- Labelled Ligand Assays
- Assays with Labelled Antibodies
- TECHNICAL DETAILS OF SOME LABELLED BINDING ASSAYS: Separation Assays with Labelled Ligands
- Labels for Immunoassays
- Fundamental Studies and the Evaluation of an Immunoassay Procedure.
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